期刊
PATHOGENS
卷 11, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/pathogens11020160
关键词
infant diarrhoea; enterotoxigenic Escherichia coli; proteomics; mucosal microbiome; regularised canonical correlation analysis; bile acid
类别
资金
- Sun Yat-sen University, China [2017181]
This study investigated the changes in mucosal microbiome and protein expression in the ileum induced by ETEC infection using pigs as a model. The results showed that ETEC infection caused inflammation, impaired bile acid circulation, and the mucosal microbiome may affect the expression of intestinal proteins.
Enterotoxigenic Escherichia coli (ETEC) is closely associated with diarrhoea in children in resource-limited countries. This study aims to investigate the change of the mucosal microbiome and protein expression in the ileum induced by E. coli K88 (ETEC) using pigs as a model. Seven weaned male pigs were orally given ETEC (1 x 10(9) CFU, n = 7), and the other seven received saline (CON, n = 7). Ileal tissues were obtained 48 hours after the ETEC challenge for both proteomic and mucosal microbiome analyses. Nine proteins were found with altered abundance between the two groups, including a decrease in FABP1 and FABP6, involved in bile acid circulation. The TLR-9 mediated pathway was also affected showing increased transcription of genes SIGIRR and MyD88. Correlations between the ileal proteins and mucosal bacterial taxa found included a positive correlation between Lactobacilllus and PPP3CA (r = 0.9, p < 0.001) and a negative correlation between Prevotella with CTNND1 (r = -0.7, p < 0.01). In conclusion, ETEC infection caused inflammation and impaired the circulation of bile acids and the mucosal microbiome may affect the expression of intestinal proteins. Further studies are needed to explain the exact roles of these affected processes in the pathogenesis of ETEC-triggered diarrhoea.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据