期刊
FRONTIERS IN MOLECULAR BIOSCIENCES
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2022.841790
关键词
amyloid; prion; octarepeat; intrinsically disordered region; protein; magic angle spinning (MAS) solid-state NMR
In this study, the influence of the dynamic domain on the structure of the amyloid core region of huPrP23-144 was systematically evaluated. It was found that deleting a large portion of the N-terminal tail can result in amyloid fibrils with native-like core structure, while deleting additional flexible residues outside the core structure causes perturbation to the fibril structure.
The Y145Stop mutant of human prion protein (huPrP23-144) is associated with a familial prionopathy and provides a convenient in vitro model for investigating amyloid strains and cross-seeding barriers. huPrP23-144 fibrils feature a compact and relatively rigid parallel in-register beta-sheet amyloid core spanning similar to 30 C-terminal amino acid residues (similar to 112-141) and a large similar to 90-residue dynamically disordered N-terminal tail domain. Here, we systematically evaluate the influence of this dynamic domain on the structure adopted by the huPrP23-144 amyloid core region, by investigating using magic-angle spinning solid-state nuclear magnetic resonance (NMR) spectroscopy a series of fibril samples formed by huPrP23-144 variants corresponding to deletions of large segments of the N-terminal tail. We find that deletion of the bulk of the N-terminal tail, up to residue 98, yields amyloid fibrils with native-like huPrP23-144 core structure. Interestingly, deletion of additional flexible residues in the stretch 99-106 located outside of the amyloid core yields shorter heterogenous fibrils with fingerprint NMR spectra that are clearly distinct from those for full-length huPrP23-144, suggestive of the onset of perturbations to the native structure and degree of molecular ordering for the core residues. For the deletion variant missing residues 99-106 we show that native huPrP23-144 core structure can be restored by seeding the fibril growth with preformed full-length huPrP23-144 fibrils.
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