The Influence of Above-Ground Herbivory on the Response of Arctic Soil Methanotrophs to Increasing CH4 Concentrations and Temperatures

Rising temperatures in the Arctic affect soil microorganisms, herbivores, and peatland vegetation, thus directly and indirectly influencing microbial CH4 production. It is not currently known how methanotrophs in Arctic peat respond to combined changes in temperature, CH4 concentration, and vegetation. We studied methanotroph responses to temperature and CH4 concentration in peat exposed to herbivory and protected by exclosures. The methanotroph activity was assessed by CH4 oxidation rate measurements using peat soil microcosms and a pure culture of Methylobacter tundripaludum SV96, qPCR, and sequencing of pmoA transcripts. Elevated CH4 concentrations led to higher CH4 oxidation rates both in grazed and exclosed peat soils, but the strongest response was observed in grazed peat soils. Furthermore, the relative transcriptional activities of different methanotroph community members were affected by the CH4 concentrations. While transcriptional responses to low CH4 concentrations were more prevalent in grazed peat soils, responses to high CH4 concentrations were more prevalent in exclosed peat soils. We observed no significant methanotroph responses to increasing temperatures. We conclude that methanotroph communities in these peat soils respond to changes in the CH4 concentration depending on their previous exposure to grazing. This conditioning influences which strains will thrive and, therefore, determines the function of the methanotroph community.

Automated summary

The study found that elevated CH4 concentrations led to increased CH4 oxidation rates in peat soils exposed to herbivory and protected by exclosures, with the strongest response observed in grazed peat soils. The relative transcriptional activities of different methanotroph community members were affected by the CH4 concentrations, with transcriptional responses to low CH4 concentrations more prevalent in grazed peat soils and responses to high CH4 concentrations more prevalent in exclosed peat soils. These findings suggest that methanotroph communities in these peat soils respond to changes in CH4 concentration based on their previous exposure to grazing.