期刊
MICROORGANISMS
卷 9, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9112336
关键词
probiotic; Lactobacillus plantarum; hydrophobicity; autoaggregation; adhesion ability
类别
资金
- Ministry of Research and Technology/National Agency for Research and Innovation (RISTEK-BRIN) through the Higher Education of Research and Development research scheme (PPUPT) [2164/UN1/DITLIT/DIT-LIT/PT/2021]
- Center of Excellence for Research and Application on Integrated Probiotic Industry, Universitas Gadjah Mada [1256/E3/PKS.04/KL/2021, 1078/UN1.P.III/DIT-LIT/PT/2021]
- [1078/UN1]
- [P.III/DIT-LIT/PT/2021]
The adhesion properties of Lactobacillus plantarum Dad-13 and Mut-7 to the intestinal mucosa of Sprague Dawley rats were studied, showing their ability to adhere to the surface of the rat intestine. Genomic analysis revealed genes related to adhesion properties in both strains, suggesting their potential role in increasing probiotics' adherence to the intestinal mucosa.
Adhesion capacity is considered one of the selection criteria for probiotic strains. The purpose of this study was to determine the adhesion properties of two candidate probiotics, Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7. The evaluation included the hydrophobicity of the cell surface using microbial adhesion to hydrocarbons (MATH), autoaggregation, and the adhesion of L. plantarum Dad-13 and L. plantarum Mut-7 to the intestinal mucosa of Sprague Dawley rat, followed by genomic analysis of the two L. plantarum strains. L. plantarum Dad-13 and L. plantarum Mut-7 showed a high surface hydrophobicity (78.9% and 83.5%) and medium autoaggregation ability (40.9% and 57.5%, respectively). The exposure of both isolates to the surface of the rat intestine increased the total number of lactic acid bacteria on the colon compartment, from 2.9 log CFU/cm(2) to 4.4 log CFU/cm(2) in L. plantarum Dad-13 treatment and to 3.86 log CFU/cm(2) in L. plantarum Mut-7 treatment. The results indicate the ability of two L. plantarum to attach to the surface of the rat intestine. The number of indigenous E. coli in the colon also decreased when the compartment was exposed to L. plantarum Dad-13 and Mut-7, from 2.9 log CFU/cm(2) to 1 log CFU/cm(2). Genomic analysis revealed that both strains have genes related to adhesion properties that could play an important role in increasing the adherence of probiotics to the intestinal mucosa such as gene encoding fibronectin-binding protein, chaperonin heat shock protein 33 (Hsp33), and genes related to the capsule and cell wall biosynthesis. Based on these findings, we believe that L. plantarum Dad-13 and L. plantarum Mut-7 have adhesion properties to the intestinal mucosa in the rat intestine model system. The present research will be essential to elucidate the molecular mechanism associated with adhesion in our two probiotic strains.
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