期刊
MICROORGANISMS
卷 9, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9122437
关键词
lactic acid bacteria; Lactococcus lactis; Weissella cibaria; synbiotics; probiotics; prebiotics; glucooligosaccharides; immunostimulatory activity
类别
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [2019R1A2C1004950]
- National Research Foundation of Korea [2019R1A2C1004950] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study confirms that synbiotics prepared by combining lactic acid bacteria with potential probiotic strains and glucooligosaccharides can significantly enhance immunostimulatory activity.
Much attention has been recently paid to the health benefits of synbiotics, a combination of probiotics and prebiotics. In this study, synbiotics were prepared by combining lactic acid bacteria with potential as probiotics and purified glucooligosaccharides, and their immunostimulatory activity was evaluated using RAW 264.7 macrophage cells. A lactic acid bacteria strain with high antioxidant activity, acid and bile salt tolerance, adhesion to Caco-2 cells, and nitric oxide (NO) production was selected as a potential probiotic strain. The selected strain, isolated from forsythia, was identified as Lactococcus lactis SG-030. The purified glucooligosaccharides produced from Weissella cibaria YRK005 were used as prebiotics. RAW 264.7 cells were treated with synbiotics in two ways. One way was a simultaneous treatment with lactic acid bacteria and glucooligosaccharides. The other way was to pre-culture the lactic acid bacteria with glucooligosaccharides followed by treatment with synbiotic culture broth or synbiotic culture supernatant. In both cases, synbiotics synergistically increased NO production in RAW 264.7 cells. In addition, synbiotics treatment increased the expression of tissue necrosis factor-alpha, interleukin (IL)-1 beta, IL-6, and inducible nitric oxide synthase genes. Synbiotics also increased the expression of P38, extracellular signal-regulated kinases, c-Jun N-terminal kinases, phosphoinositide 3-kinase, and Akt proteins. The results confirmed that the synbiotics prepared in this study exhibited synergistic immunostimulatory activity.
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