期刊
MICROORGANISMS
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms10010021
关键词
bacterial endophytes; wheat varieties; phylogeny; biochemical analysis; antimicrobial activity; stress tolerance; plant growth-promoting ability
类别
Endophytic bacteria isolated from Pakistani wheat seeds showed beneficial characteristics such as nitrogen fixation, phosphorus solubilization, indole-3-acetic acid production, and antimicrobial activity. The GI-6 strain exhibited strong salt tolerance and growth-promoting effects on wheat plants.
Endophytic bacteria have been utilized as an alternative source to chemical fertilizers and pesticides to enhance plant productivity and defense mechanisms against biotic and abiotic stress. Five endophytic bacterial strains were isolated from the seeds of three different Pakistani wheat varieties (Ghaneemat-e-IBGE, Atta-Habib, and Siren). The isolated strains AH-1, S-5, S-7, GI-1, and GI-6 showed phylogenetic similarity with Bacillus altitudinis, B. aryabhattai, B. wiedmannii, Pseudomonas aeruginosa, and Burkholderia gladioli, respectively. All strains showed catalase activity (except AH-1) and Indole-3-acetic acid production, with the highest concentration (16.77 mu g center dot mL(-1)) found for GI-6, followed by S-5 (11.5 mu g center dot mL(-1)), nitrogen assimilation (except S-7), phosphorus solubilization (except S-7 and AH-1), and ability to produce siderophores, with maximum productions for GI-6 (31 +/- 3.5 psu) and GI-1 (30 +/- 2.9 psu). All five analyzed strains possessed antimicrobial activity, which was particularly strong in GI-6 and S-5 against Klebsiella pneumonia, Escherichia coli, and Bacillus subtilis. Increasing salinity stress with NaCl negatively affected the bacterial growth of all isolates. However, strains GI-6 and S-5 showed salt tolerance after three days of incubation. A drought tolerance test resulted in a negative impact of poly ethylene glycol on bacterial growth, which was, however, less pronounced in GI-6 strain. The GI-6 strain revealed growth-promoting effects on inoculated wheat plants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据