期刊
MICROORGANISMS
卷 9, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9112284
关键词
aptamer; Parabacteroides distasonis; FluCell-SELEX; biosensor development
类别
资金
- China Scholarship Council [202108080084]
- Ministry of Science, Research and Arts of the state of Baden-Wurttemberg
- Baden-Wurttemberg Stiftung [BiofMO_005]
- European Union [686271]
- Ministry of Science, Research and the Arts of Baden-Wurttemberg (MWK) [Forderkennzeichen: 7533-10-5-186A, 7533-10-5-190]
This study reports on the evolution of a high-affinity aptamer library using FluCell-SELEX technology, which can specifically recognize, bind and label P. distasonis, and accurately distinguish P. distasonis from other gut bacteria in mixtures.
Single-stranded DNA aptamers as affinity molecules for the rapid, reliable detection of intestinal bacteria are of particular interest to equip health systems with novel robust and cheap diagnostic tools for monitoring the success of supplementation strategies with selected probiotic gut bacteria in the fight against major widespread threats, such as obesity and neurodegenerative diseases. The human gut bacterium Parabacteroides distasonis (P. distasonis) is positively associated with diseases such as obesity, non-alcoholic fatty liver disease and multiple sclerosis with reduced cell counts in these diseases and is thus a promising potential probiotic bacterium for future microbial supplementation. In this paper we report on the evolution of a specific polyclonal aptamer library by the fluorescence based FluCell-SELEX directed against whole cells of P. distasonis that specifically and efficiently binds and labels P. distasonis. The aptamer library showed high binding affinity and was suited to quantitatively discriminate P. distasonis from other prominent gut bacteria also in mixtures. We believe that this library against a promising probiotic bacterium as a prototype may open new routes towards the development of novel biosensors for the easy and efficient quantitative monitoring of microbial abundance in human microbiomes in general.
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