期刊
MICROORGANISMS
卷 9, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9102142
关键词
pectin; rhamnogalacturonan I; Bifidobacteriaceae; Bacteroides dorei; acetate; propionate; in vitro
类别
资金
- NutriLeads B.V., Wageningen, The Netherlands
- European Union's Horizon 2020 research and innovation programme [811592]
- Eurostars [E! 10574-NIMF]
- Federal Public Planning Service-Science Policy, Belgium
This study demonstrates the prebiotic potential of a carrot-derived pectin extract enriched for rhamnogalacturonan I (cRG-I) on the human gut microbiome, showing consistent effects on certain bacterial species and metabolites. The findings suggest a specialized consortium is involved in the fermentation of cRG-I, indicating potential benefits for gut health.
The human gut microbiome is currently recognized to play a vital role in human biology and development, with diet as a major modulator. Therefore, novel indigestible polysaccharides that confer a health benefit upon their fermentation by the microbiome are under investigation. Based on the recently demonstrated prebiotic potential of a carrot-derived pectin extract enriched for rhamnogalacturonan I (cRG-I), the current study aimed to assess the impact of cRG-I upon repeated administration using the M-SHIME technology (3 weeks at 3g cRG-I/d). Consistent effects across four simulated adult donors included enhanced levels of acetate (+21.1 mM), propionate (+17.6 mM), and to a lesser extent butyrate (+4.1 mM), coinciding with a marked increase of OTUs related to Bacteroides dorei and Prevotella species with versatile enzymatic potential likely allowing them to serve as primary degraders of cRG-I. These Bacteroidetes members are able to produce succinate, explaining the consistent increase of an OTU related to the succinate-converting Phascolarctobacterium faecium (+0.47 log(10)(cells/mL)). While the Bifidobacteriaceae family remained unaffected, a specific OTU related to Bifidobacterium longum increased significantly upon cRG-I treatment (+1.32 log(10)(cells/mL)). Additional monoculture experiments suggested that Bifidobacterium species are unable to ferment cRG-I structures as such and that B. longum probably feeds on arabinan and galactan side chains of cRG-I, released by aforementioned Bacteroidetes members. Overall, this study confirms the prebiotic potential of cRG-I and additionally highlights the marked consistency of the microbial changes observed across simulated subjects, suggesting the involvement of a specialized consortium in cRG-I fermentation by the human gut microbiome.
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