期刊
MICROORGANISMS
卷 9, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9122581
关键词
waste-activated sludge; full-scale plant; anaerobic digestion; 16S rRNA gene sequencing; microbial population dynamics
类别
The study found that the majority of the reads in the anaerobic digester were affiliated with fermentative bacteria, followed by an increase in the proportion of Bacteroidetes over time and Methanosaeta as the most abundant methanogenic component. Specific quantitative data from real-time PCR showed an enrichment of bacteria and methanogens once the steady state was reached.
Anaerobic digestion is a widely used technology for sewage sludge stabilization and biogas production. Although the structure and composition of the microbial communities responsible for the process in full-scale anaerobic digesters have been investigated, little is known about the microbial successional dynamics during the start-up phase and the response to variations occurring in such systems under real operating conditions. In this study, bacterial and archaeal population dynamics of a full-scale mesophilic digester treating activated sludge were investigated for the first time from the start-up, performed without adding external inoculum, to steady-state operation. High-throughput 16S rRNA gene sequencing was used to describe the microbiome evolution. The large majority of the reads were affiliated to fermentative bacteria. Bacteroidetes increased over time, reaching 22% of the total sequences. Furthermore, Methanosaeta represented the most abundant methanogenic component. The specific quantitative data generated by real-time PCR indicated an enrichment of bacteria and methanogens once the steady state was reached. The analysis allowed evaluation of the microbial components more susceptible to the shift from aerobic to anaerobic conditions and estimation of the microbial components growing or declining in the system. Additionally, activated sludge was investigated to evaluate the microbial core selected by the WWTP operative conditions.
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