期刊
MICROORGANISMS
卷 10, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms10020216
关键词
Succulent Karoo Biome; legumes species; diazotrophs; metabarcoding; rhizosphere soils; 16S rRNA gene; nifH gene
类别
资金
- DSI-NRF Centre of Excellence in Plant Health and Biotechnology (CPHB) through the Forestry and Agricultural Biotechnology Institute (FABI),University of Pretoria, South Africa
This study assessed the bacterial diversity in the rhizosphere soils of native and encroaching legumes in the Succulent Karoo Biome of South Africa. The results showed a diverse range of bacteria, including diazotrophic bacteria, that support plant growth in harsh environments. Vachellia karroo had the highest diversity and influence on soil microbes among the legume species studied.
Total and diazotrophic bacteria were assessed in the rhizosphere soils of native and encroaching legumes growing in the Succulent Karoo Biome (SKB), South Africa. These were Calobota sericea, Lessertia diffusa, Vachellia karroo, and Wiborgia monoptera, of Fabaceae family near Springbok (Northern Cape Province) and neighboring refugia of the Fynbos biome for C. sericea for comparison purposes. Metabarcoding approach using 16S rRNA gene revealed Actinobacteria (26.7%), Proteobacteria (23.6%), Planctomycetes, and Acidobacteria (10%), while the nifH gene revealed Proteobacteria (70.3%) and Cyanobacteria (29.5%) of the total sequences recovered as the dominant phyla. Some of the diazotrophs measured were assigned to families; Phyllobacteriaceae (39%) and Nostocaceae (24.4%) (all legumes), Rhodospirillaceae (7.9%), Bradyrhizobiaceae (4.6%) and Methylobacteriaceae (3%) (C. sericea, V. karroo, W. monoptera), Rhizobiaceae (4.2%; C. sericea, L. diffusa, V. Karroo), Microchaetaceae (4%; W. monoptera, V. karroo), Scytonemataceae (3.1%; L. diffusa, W. monoptera), and Pseudomonadaceae (2.7%; V. karroo) of the total sequences recovered. These families have the potential to fix the atmospheric nitrogen. While some diazotrophs were specific or shared across several legumes, a member of Mesorhizobium species was common in all rhizosphere soils considered. V. karroo had statistically significantly higher Alpha and distinct Beta-diversity values, than other legumes, supporting its influence on soil microbes. Overall, this work showed diverse bacteria that support plant life in harsh environments such as the SKB, and shows how they are influenced by legumes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据