期刊
MICROORGANISMS
卷 9, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9112276
关键词
enterocin F4-9; glycocin; bacteriocin; biosynthesis; glycosylation
类别
资金
- Egyptian postdoctoral scholarship
- JSPS [17H03797, 21H02107]
- Grants-in-Aid for Scientific Research [21H02107, 17H03797] Funding Source: KAKEN
In this study, the biosynthetic gene cluster of Enterocin F4-9 was cloned and expressed in Enterococcus faecalis JH2-2, resulting in enhanced antimicrobial activity against both Gram-positive and Gram-negative bacteria. The removal of the N-terminal extension led to increased potency of the peptide, highlighting a novel biosynthetic mechanism of glycocins.
Enterocin F4-9 belongs to the glycocin family having post-translational modifications by two molecules of N-acetylglucosamine beta-O-linked to Ser37 and Thr46. In this study, the biosynthetic gene cluster of enterocin F4-9 was cloned and expressed in Enterococcus faecalis JH2-2. Production of glycocin by the JH2-2 expression strain was confirmed by expression of the five genes. The molecular weight was greater than glycocin secreted by the wild strain, E. faecalis F4-9, because eight amino acids from the N-terminal leader sequence remained attached. This N-terminal extension was eliminated after treatment with the culture supernatant of strain F4-9, implying an extracellular protease from E. faecalis F4-9 cleaves the N-terminal sequence. Thus, leader sequences cleavage requires two steps: the first via the EnfT protease domain and the second via extracellular proteases. Interestingly, the long peptide, with N-terminal extension, demonstrated advanced antimicrobial activity against Gram-positive and Gram-negative bacteria. Furthermore, enfC was responsible for glycosylation, a necessary step prior to secretion and cleavage of the leader peptide. In addition, enfI was found to grant self-immunity to producer cells against enterocin F4-9. This report demonstrates specifications of the minimal gene set responsible for production of enterocin F4-9, as well as a new biosynthetic mechanism of glycocins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据