期刊
ANTIOXIDANTS
卷 10, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/antiox10101589
关键词
antiglycation; advanced glycation end products; glycation stress; Pholiota nameko; polysaccharide; human dermal fibroblasts; cell aging
资金
- Ministry of Science and Technology, Republic of China [MOST 109-2622-B-005-003-CC2]
Pholiota nameko polysaccharides (PNPs) show strong antiglycation ability, effectively reducing oxidative stress and cell damage caused by glycation, and protecting cells from further harm.
Advanced glycation end products (AGEs) can induce oxidative stress and inflammation. AGEs are major risk factors for the development of many aging-related diseases, such as cancer and diabetes. In this study, Pholiota nameko polysaccharides (PNPs) were prepared from water extract of P. nameko via graded alcohol precipitation (40%, 60%, and 80% v/v). We explored the in vitro antiglycation ability of the PNPs and inhibition of methylglyoxal (MG)-induced Hs68 cell damage. In a bovine serum albumin (BSA) glycation system, PNPs significantly inhibited the formation of Amadori products. Fluorescence spectrophotometry revealed that the PNPs trapped MG and reduced MG-induced changes in functional groups (carbonyl and epsilon-NH2) in the BSA. Pretreating Hs68 cells with PNPs enhanced the cell survival rate and protected against MG-induced cell damage. This was due to decreased intracellular ROS content. PNPs thus mitigate skin cell damage and oxidative stress resulting from glycation stress, making them a potential raw material for antiaging-related skincare products.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据