期刊
BIOMOLECULES
卷 11, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/biom11111735
关键词
Cellulosimicrobium funkei; intestinal symbiont; Eisenia fetida; GH19; endo-type chitinase
资金
- KRIBB Research Initiative Program [KGM5492113]
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) [2016M3A9A5922628]
- [LCM0042011]
- National Research Foundation of Korea [2016M3A9A5922628] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study identified a novel endo-type chitinase ChiJ with high activity, particularly towards ethylene glycol chitin, making it a suitable biocatalyst for preparing N,N'-diacetyl-beta-d-chitobiose, which has shown potential in alleviating metabolic dysfunction associated with type 2 diabetes.
Endo-type chitinase is the principal enzyme involved in the breakdown of N-acetyl-d-glucosamine-based oligomeric and polymeric materials through hydrolysis. The gene (966-bp) encoding a novel endo-type chitinase (ChiJ), which is comprised of an N-terminal chitin-binding domain type 3 and a C-terminal catalytic glycoside hydrolase family 19 domain, was identified from a fibrolytic intestinal symbiont of the earthworm Eisenia fetida, Cellulosimicrobium funkei HY-13. The highest endochitinase activity of the recombinant enzyme (rChiJ: 30.0 kDa) toward colloidal shrimp shell chitin was found at pH 5.5 and 55 & DEG;C and was considerably stable in a wide pH range (3.5-11.0). The enzyme exhibited the highest biocatalytic activity (338.8 U/mg) toward ethylene glycol chitin, preferentially degrading chitin polymers in the following order: ethylene glycol chitin > colloidal shrimp shell chitin > colloidal crab shell chitin. The enzymatic hydrolysis of N-acetyl-beta-d-chitooligosaccharides with a degree of polymerization from two to six and colloidal shrimp shell chitin yielded primarily N,N & PRIME;-diacetyl-beta-d-chitobiose together with a small amount of N-acetyl-d-glucosamine. The high chitin-degrading ability of inverting rChiJ with broad pH stability suggests that it can be exploited as a suitable biocatalyst for the preparation of N,N & PRIME;-diacetyl-beta-d-chitobiose, which has been shown to alleviate metabolic dysfunction associated with type 2 diabetes.
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