期刊
VACCINES
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/vaccines10010067
关键词
oral vaccine; Salmonella; SARS-CoV-2; spike protein
This study evaluated the potential of an oral bacteria-based vaccine as a safe and effective platform for large-scale, long-term immunization. Engineered Salmonella cells expressing viral proteins related to COVID-19 were used to develop an oral vaccine candidate, which showed promising results in promoting antigen-specific antibody production and inducing cellular immunity. These findings suggest that Salmonella strains could be a valuable platform for developing an oral vaccine alternative to combat COVID-19 and future infectious disease outbreaks.
Effective vaccine development for global outbreaks, such as the coronavirus disease 2019 (COVID-19), has been successful in the short run. However, the currently available vaccines have been associated with a higher frequency of adverse effects compared with other general vaccines. In this study, the possibility of an oral bacteria-based vaccine that can be safely used as a platform for large-scale, long-term immunization was evaluated. A well-known Salmonella strain that was previously considered as a vaccine delivery candidate was used. Recombinant Salmonella cells expressing engineered viral proteins related with COVID-19 pathogenesis were engineered, and the formulation of the oral vaccine candidate strain was evaluated by in vitro and in vivo experiments. First, engineered S proteins were synthesized and cloned into expression vectors, which were than transformed into Salmonella cells. In addition, when orally administrated to mice, the vaccine promoted antigen-specific antibody production and cellular immunity was induced with no significant toxicity effects. These results suggest that Salmonella strains may represent a valuable platform for the development of an oral vaccine for COVID-19 as an alternative to tackle the outbreak of various mutated coronavirus strains and new infectious diseases in the future.
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