4.7 Review

Review on Strategies and Technologies for Exosome Isolation and Purification

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Rapid isolation and proteome analysis of urinary exosome based on double interactions of Fe3O4@TiO2-DNA aptamer

Ning Zhang et al.

Summary: Proteins carried by exosomes in urine are potential biomarkers or therapeutic carriers for diseases, making the isolation of exosomes crucial for protein analysis. A new biocompatible material, Fe3O4@TiO2-CD63 aptamer, was designed to efficiently isolate exosomes from urine, capturing 92.6% intact exosomes within 10 minutes and detecting 999 proteins through LC-MS/MS.

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Extracellular Vesicles in Liquid Biopsies: Potential for Disease Diagnosis

Jialing Liu et al.

Summary: Liquid biopsy using extracellular vesicles (EVs) is a promising tool for early cancer diagnosis, allowing for genomic, proteomic, and lipidomic analyses. EVs are nanosized bilayer particles secreted by cells, carrying various cargoes and protecting them from degradation in the extracellular environment. This review provides a comprehensive introduction to the properties and applications of EVs in liquid biopsy diagnostics.

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Article Multidisciplinary Sciences

Acoustofluidic centrifuge for nanoparticle enrichment and separation

Yuyang Gu et al.

Summary: The acoustofluidic centrifugation technique combines acoustic wave actuation with fluidic droplet spinning to achieve rapid nanoparticle enrichment and separation. It demonstrates a high resolution in processing biological samples, being able to identify and isolate exosome subpopulations.

SCIENCE ADVANCES (2021)

Article Biochemistry & Molecular Biology

ExoCAS-2: Rapid and Pure Isolation of Exosomes by Anionic Exchange Using Magnetic Beads

Hyunsung Kim et al.

Summary: Extracellular vesicles are important biomarkers in liquid biopsies, but current EV-isolation technologies are limited. This study introduces a magnetic bead-based ion exchange platform, ExoCAS-2, for isolating EVs with high purity and yield within 40 minutes. Compared to existing methods, this platform shows superior performance in terms of operation time, purity, and recovery rate, highlighting its potential for isolating exosomes from blood plasma.

BIOMEDICINES (2021)

Article Biochemistry & Molecular Biology

Exosomes isolated from two different cell lines using three different isolation techniques show variation in physical and molecular characteristics

Manish Dash et al.

Summary: The study compared three major exosome isolation techniques, TEI, PROSPR, and UC, for their effectiveness in isolating exosomes from cancer cell conditioned media. Through multiple characterization and analysis methods, the study identified advantages, disadvantages, and suitable applications for each technique.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2021)

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Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries

Hyuna Sung et al.

Summary: The global cancer burden in 2020 saw an estimated 19.3 million new cancer cases and almost 10.0 million cancer deaths. Female breast cancer surpassed lung cancer as the most commonly diagnosed cancer, while lung cancer remained the leading cause of cancer death. These trends are expected to rise in 2040, with transitioning countries experiencing a larger increase compared to transitioned countries due to demographic changes and risk factors associated with globalization and a growing economy. Efforts to improve cancer prevention measures and provision of cancer care in transitioning countries will be crucial for global cancer control.

CA-A CANCER JOURNAL FOR CLINICIANS (2021)

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Small extracellular vesicles in cancer

Komal Abhange et al.

Summary: Extracellular vesicles (EVs) are lipid-bilayer enclosed vesicles released from cells, carrying various molecules and playing crucial roles in tumors. EVs exhibit expression patterns similar to parental cells, aiding in early cancer diagnosis and treatment monitoring. In addition to research and diagnostics, EVs are utilized as drug delivery nanocarriers and therapeutic targets.

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Ultrasensitive bioassaying of HER-2 protein for diagnosis of breast cancer using reduced graphene oxide/chitosan as nanobiocompatible platform

Hassan Nasrollahpour et al.

Summary: A label-free bioassay using an ECL immunosensor was developed for quantifying breast cancer through HER-2 protein, embedding [Ru(bpy)(3)](2+) into biocompatible chitosan and utilizing reduced graphene oxide as a substrate for increased sensitivity and signal stability. The designed immunosensor showed promising results for detecting HER-2 protein in breast cancer patients, demonstrating potential for use in biomedical laboratories for early detection.

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Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays

Hui-Yu Liu et al.

Summary: A novel EV-capture strategy based on supported lipid membrane microarrays is proposed, enabling highly selective and efficient capture of EVs. The platform offers rapid capture, high specificity, and sensitivity, with only a small need in analyte volume. The nucleic acid cargo of captured EVs is retained on the lipid array, providing potential for downstream analysis.

ADVANCED MATERIALS (2021)

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Emerging technologies and commercial products in exosome-based cancer diagnosis and prognosis

Mehdi Mohammadi et al.

Summary: This study provides an overview of the role of exosomes in cancer diagnosis and prognosis, investigates emerging exosome technologies, evaluates the advantages and drawbacks of different technologies, and discusses their clinical challenges for cancer diagnosis and prognosis. Commercial platforms for exosomal detection and analysis are introduced, with a focus on their performance and impact on cancer diagnosis and prognosis, as well as the risks associated with the development of next-generation exosome devices.

BIOSENSORS & BIOELECTRONICS (2021)

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A Holistic Review of the State-of-the-Art Microfluidics for Exosome Separation: An Overview of the Current Status, Existing Obstacles, and Future Outlook

Lihua Ding et al.

Summary: Exosomes are small extracellular vesicles secreted by various cells into body fluids and hold great potential for disease diagnosis and therapy. However, isolating exosomes is challenging due to their heterogeneity and the complexity of bio-fluids. Recent advancements in microfluidic technologies offer an efficient system for exosome extraction, showcasing high speeds, portability, and low sample volumes.
Review Biochemistry & Molecular Biology

Circular RNAs Are Promising Biomarkers in Liquid Biopsy for the Diagnosis of Non-small Cell Lung Cancer

Lanxiang Huang et al.

Summary: The high incidence and mortality of lung cancer make early detection crucial, with circRNAs playing a critical role in cancer progression and showing potential as specific diagnostic markers for NSCLC. Liquid biopsy, with its high sensitivity and possibility of multiple sampling, is essential in early cancer detection.

FRONTIERS IN MOLECULAR BIOSCIENCES (2021)

Article Cell & Tissue Engineering

Defined MSC exosome with high yield and purity to improve regenerative activity

Jun Yong Kim et al.

Summary: This study compared the isolation yield and purity of exosomes using ultracentrifuge-based and tangential flow filtration-based methods, showing that the TFF method had higher yield and significantly improved purity. Highly purified exosomes from UCMSCs demonstrated enhanced wound healing and angiogenic effects with HCAEC, suggesting that defined MSC exosomes with high yield and purity could increase regenerative activity.

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Earlier diagnosis: the importance of cancer symptoms

Katriina Whitaker

LANCET ONCOLOGY (2020)

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Cancer Liquid Biopsy Using Integrated Microfluidic Exosome Analysis Platforms

Guiying Li et al.

BIOTECHNOLOGY JOURNAL (2020)

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Unraveling the mechanisms that specify molecules for secretion in extracellular vesicles

Andrew M. Leidal et al.

METHODS (2020)

Review Multidisciplinary Sciences

The biology, function, and biomedical applications of exosomes

Raghu Kalluri et al.

SCIENCE (2020)

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Extracellular Vesicles Could Carry an Evolutionary Footprint in Interkingdom Communication

Ricardo Correa et al.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2020)

Article Biochemical Research Methods

Characterization of Urinary Exosomes Purified with Size Exclusion Chromatography and Ultracentrifugation

Sheng Guan et al.

JOURNAL OF PROTEOME RESEARCH (2020)

Article Multidisciplinary Sciences

Non-invasive early detection of cancer four years before conventional diagnosis using a blood test

Xingdong Chen et al.

NATURE COMMUNICATIONS (2020)

Review Oncology

Exosomes as a Multicomponent Biomarker Platform in Cancer

Valerie S. LeBleu et al.

TRENDS IN CANCER (2020)

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Ultrasensitive detection of circulating exosomes with a 3D-nanopatterned microfluidic chip

Peng Zhang et al.

NATURE BIOMEDICAL ENGINEERING (2019)

Review Chemistry, Medicinal

Exosome manufacturing status

William Whitford et al.

FUTURE MEDICINAL CHEMISTRY (2019)

Review Chemistry, Analytical

Microfluidic Technology for Clinical Applications of Exosomes

Florina S. Iliescu et al.

MICROMACHINES (2019)

Review Medical Laboratory Technology

Biosensors for early diagnosis of pancreatic cancer: a review

Lisheng Qian et al.

TRANSLATIONAL RESEARCH (2019)

Review Medical Laboratory Technology

Exosomes in inflammation and role as biomarkers

Lara Console et al.

CLINICA CHIMICA ACTA (2019)

Article Medicine, Research & Experimental

A highly efficient method for isolating urinary exosomes

Liuqing He et al.

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019)

Review Chemistry, Multidisciplinary

New Technologies for Analysis of Extracellular Vesicles

Huilin Shao et al.

CHEMICAL REVIEWS (2018)

Review Oncology

Exosomes as a liquid biopsy for lung cancer

Shaohua Cui et al.

LUNG CANCER (2018)

Review Pharmacology & Pharmacy

Biogenesis and function of extracellular vesicles in cancer

Maarten P. Bebelman et al.

PHARMACOLOGY & THERAPEUTICS (2018)

Article Multidisciplinary Sciences

Exosome isolation from distinct biofluids using precipitation and column-based approaches

Tania Soares Martins et al.

PLOS ONE (2018)

Review Biochemistry & Molecular Biology

Manufacturing Exosomes: A Promising Therapeutic Platform

Ivano Luigi Colao et al.

TRENDS IN MOLECULAR MEDICINE (2018)

Article Ophthalmology

Liquid Biopsy of Vitreous Reveals an Abundant Vesicle Population Consistent With the Size and Morphology of Exosomes

Yuanjun Zhao et al.

TRANSLATIONAL VISION SCIENCE & TECHNOLOGY (2018)

Review Oncology

The Origin and Functions of Exosomes in Cancer

Chitra Rajagopal et al.

FRONTIERS IN ONCOLOGY (2018)

Review Chemistry, Physical

Methods and Technologies for Exosome Isolation and Characterization

Mengdi Zhang et al.

SMALL METHODS (2018)

Review Biochemistry & Molecular Biology

Current knowledge on exosome biogenesis and release

Nina Pettersen Hessvik et al.

CELLULAR AND MOLECULAR LIFE SCIENCES (2018)

Article Multidisciplinary Sciences

Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer

Sushrut Kamerkar et al.

NATURE (2017)

Article Multidisciplinary Sciences

Isolation of exosomes from whole blood by integrating acoustics and microfluidics

Mengxi Wu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)

Review Biochemistry & Molecular Biology

Lipids in exosomes: Current knowledge and the way forward

Tore Skotland et al.

PROGRESS IN LIPID RESEARCH (2017)

Review Cell & Tissue Engineering

Concise Review: MSC-Derived Exosomes for Cell-Free Therapy

Donald G. Phinney et al.

STEM CELLS (2017)

Review Medicine, Research & Experimental

Progress in Exosome Isolation Techniques

Pin Li et al.

THERANOSTICS (2017)

Article Cell Biology

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper

Bogdan Mateescu et al.

JOURNAL OF EXTRACELLULAR VESICLES (2017)

Review Cardiac & Cardiovascular Systems

Methodological Guidelines to Study Extracellular Vesicles

Frank A. W. Coumans et al.

CIRCULATION RESEARCH (2017)

Review Spectroscopy

Mass spectrometry of extracellular vesicles

Gabriella Pocsfalvi et al.

MASS SPECTROMETRY REVIEWS (2016)

Article Engineering, Biomedical

Efficient production and enhanced tumor delivery of engineered extracellular vesicles

Dionysios C. Watson et al.

BIOMATERIALS (2016)

Article Medicine, Research & Experimental

Charge-based precipitation of extracellular vesicles

Maria Chiara Deregibus et al.

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2016)

Article Neurosciences

Enrichment of extracellular vesicles from tissues of the central nervous system by PROSPR

Xavier Gallart-Palau et al.

MOLECULAR NEURODEGENERATION (2016)

Article Nanoscience & Nanotechnology

Nanoscale lateral displacement arrays for the separation of exosomes and colloids down to 20 nm

Benjamin H. Wunsch et al.

NATURE NANOTECHNOLOGY (2016)

Article Chemistry, Analytical

Isolation of extracellular vesicle from blood plasma using electrophoretic migration through porous membrane

Siwoo Cho et al.

SENSORS AND ACTUATORS B-CHEMICAL (2016)

Article Multidisciplinary Sciences

Residual matrix from different separation techniques impacts exosome biological activity

Lucia Paolini et al.

SCIENTIFIC REPORTS (2016)

Review Cell Biology

Exosomal miRNAs as cancer biomarkers and therapeutic targets

Arron Thind et al.

JOURNAL OF EXTRACELLULAR VESICLES (2016)

Editorial Material Chemistry, Medicinal

Exosomal MicroRNAs in Tissue Crosstalk

David Gurwitz

DRUG DEVELOPMENT RESEARCH (2015)

Article Chemistry, Multidisciplinary

Using exosomes, naturally-equipped nanocarriers, for drug delivery

Elena V. Batrakova et al.

JOURNAL OF CONTROLLED RELEASE (2015)

Article Biochemical Research Methods

Antibody-free isolation of rare cancer cells from blood based on 3D lateral dielectrophoresis

I-Fang Cheng et al.

LAB ON A CHIP (2015)

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Glypican-1 identifies cancer exosomes and detects early pancreatic cancer

Sonia A. Melo et al.

NATURE (2015)

Review Genetics & Heredity

Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function

Jian Zhang et al.

GENOMICS PROTEOMICS & BIOINFORMATICS (2015)

Review Cell Biology

Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles

Marina Colombo et al.

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 30 (2014)

Review Biochemistry & Molecular Biology

Exosomes as new vesicular lipid transporters involved in cell-cell communication and various pathophysiologies

Michel Record et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS (2014)

Letter Cell Biology

Double-stranded DNA in exosomes: a novel biomarker in cancer detection

Caitlin Williams et al.

CELL RESEARCH (2014)

Article Biochemical Research Methods

A novel salting-out procedure for the isolation of tumor-derived exosomes

Zachary Brownlee et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2014)

Article Cell Biology

Comparative analysis of discrete exosome fractions obtained by differential centrifugation

Dennis K. Jeppesen et al.

JOURNAL OF EXTRACELLULAR VESICLES (2014)

Review Cell Biology

Extracellular vesicles: Exosomes, microvesicles, and friends

Graca Raposo et al.

JOURNAL OF CELL BIOLOGY (2013)

Article Biotechnology & Applied Microbiology

Extracellular vesicles: biology and emerging therapeutic opportunities

Samir EL Andaloussi et al.

NATURE REVIEWS DRUG DISCOVERY (2013)

Review Genetics & Heredity

Signaling Pathways in Exosomes Biogenesis, Secretion and Fate

Lorena Urbanelli et al.

Article Biochemistry & Molecular Biology

Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy

Yi Lee et al.

HUMAN MOLECULAR GENETICS (2012)

Article Cell Biology

Syndecan-syntenin-ALIX regulates the biogenesis of exosomes

Maria Francesca Baietti et al.

NATURE CELL BIOLOGY (2012)

Article Multidisciplinary Sciences

On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves

Xiaoyun Ding et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Review Pharmacology & Pharmacy

Classification, Functions, and Clinical Relevance of Extracellular Vesicles

Edwin van der Pol et al.

PHARMACOLOGICAL REVIEWS (2012)

Review Immunology

The Roles of Tumor-Derived Exosomes in Cancer Pathogenesis

Chenjie Yang et al.

CLINICAL & DEVELOPMENTAL IMMUNOLOGY (2011)

Review Biochemical Research Methods

Exosomes: Extracellular organelles important in intercellular communication

Suresh Mathivanan et al.

JOURNAL OF PROTEOMICS (2010)

Article Biochemical Research Methods

Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes

Hong Ji et al.

ELECTROPHORESIS (2008)

Article Multidisciplinary Sciences

Ceramide triggers budding of exosome vesicles into multivesicular Endosomes

Katarina Trajkovic et al.

SCIENCE (2008)

Review Immunology

Exosomes: From biogenesis and secretion to biological function

Sascha Keller et al.

IMMUNOLOGY LETTERS (2006)

Article Multidisciplinary Sciences

Endosomal compartmentalization in three dimensions: Implications for membrane fusion

JLAN Murk et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)

Article Biochemical Research Methods

Production and characterization of clinical grade exosomes derived from dendritic cells

HG Lamparski et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2002)

Review Oncology

Dissemination and growth of cancer cells in metastatic sites

AF Chambers et al.

NATURE REVIEWS CANCER (2002)

Review Immunology

Exosomes:: Composition, biogenesis and function

C Théry et al.

NATURE REVIEWS IMMUNOLOGY (2002)