期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.794742
关键词
cell surface display; yeast; anchor; microbial engineering; whole cell biocatalyst
资金
- National Council of Science and Technology (CONACyT) [CONACyT CB 2017-2018 A1-S-34559]
- CONACyT [558865]
Yeast surface display (YSD) is a whole-cell platform for the heterologous expression of proteins immobilized on the yeast's cell surface. Recent strategies aim to improve the efficiency and selection of displayed proteins. YSD is considered as a cutting-edge technology for vectorial expression of proteins and peptides.
Yeast surface display (YSD) is a whole-cell platform used for the heterologous expression of proteins immobilized on the yeast's cell surface. YSD combines the advantages eukaryotic systems offer such as post-translational modifications, correct folding and glycosylation of proteins, with ease of cell culturing and genetic manipulation, and allows of protein immobilization and recovery. Additionally, proteins displayed on the surface of yeast cells may show enhanced stability against changes in temperature, pH, organic solvents, and proteases. This platform has been used to study protein-protein interactions, antibody design and protein engineering. Other applications for YSD include library screening, whole-proteome studies, bioremediation, vaccine and antibiotics development, production of biosensors, ethanol production and biocatalysis. YSD is a promising technology that is not yet optimized for biotechnological applications. This mini review is focused on recent strategies to improve the efficiency and selection of displayed proteins. YSD is presented as a cutting-edge technology for the vectorial expression of proteins and peptides. Finally, recent biotechnological applications are summarized. The different approaches described herein could allow for a better strategy cascade for increasing protein/peptide interaction and production.
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