4.7 Article

Multifunctional Gold Nano-Cytosensor With Quick Capture, Electrochemical Detection, and Non-Invasive Release of Circulating Tumor Cells for Early Cancer Treatment

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.783661

关键词

CTCs; early diagnosis and treatment; electrochemical cytosensor; non-invasive release; multifunctional Au nanoparticles

资金

  1. National Key R&D Program of China [2020YFC2004500]
  2. National Natural Science Foundation of China [81771982, 91959112, 21803075, 81902166]
  3. Science Foundation of the Chinese Academy of Sciences [2020SYHZ0041]
  4. Instrument Developing Project of Chinese Academy of Science [YJKYYQ20200038]
  5. Science and Technology Department of Jinan City [2018GXRC016]

向作者/读者索取更多资源

CTCs, shed from solid primary tumors into the blood, increase the risk of metastasis and recurrence. The developed ultra-sensitive electrochemical cytosensor can capture, detect, and release EpCAM-positive tumor cells with good stability and detection accuracy.
Circulating tumor cells (CTCs) are metastatic tumor cells that shed into the blood from solid primary tumors, and their existence significantly increases the risk of metastasis and recurrence. The timely discovery and detection of CTCs are of considerable importance for the early diagnosis and treatment of metastasis. However, the low number of CTCs hinders their detection. In the present study, an ultrasensitive electrochemical cytosensor for specific capture, quantitative detection, and noninvasive release of EpCAM-positive tumor cells was developed. The biosensor was manufactured using gold nanoparticles (AuNPs) to modify the electrode. Three types of AuNPs with controllable sizes and conjugated with a targeting molecule of monoclonal anti-EpCAM antibody were used in this study. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) of the cytosensors were performed to evaluate the cell capture efficiency and performance. The captured 4T1 cells by the AuNPs hindered electron transport efficiency, resulting in increased EIS responses. The cell capture response recorded using EIS or DPV indicated that the optimal AuNPs size should be 17 nm. The cell capture response changed linearly with the concentration range from 8.0 x 10 to 1 x 10(7) cells/mL, and the limit of detection was 50 cells/mL. After these measurements, glycine-HCl (Gly-HCl) was used as an antibody eluent to destroy the binding between antigen and antibody to release the captured tumor cells without compromising their viability for further clinical research. This protocol realizes rapid detection of CTCs with good stability, acceptable assay precision, significant fabrication reproducibility with a relative standard deviation of 2.09%, and good recovery of cells. Our results indicate that the proposed biosensor is promising for the early monitoring of CTCs and may help customize personalized treatment options.

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