期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.787881
关键词
circular RNA; RNA synthesis; in vitro transcription; enzymatic ligation; ligase; permuted intron-exon
资金
- Beijing Natural Science Foundation [2192023]
- National Natural Science Foundation of China [21878173]
- National Key R&D Program of China [2018YFA0901700]
circRNA, a unique noncoding RNA molecule abundant in cells, has diverse functions such as miRNA sponge and protein scaffold. It plays a significant role in medical applications like circRNA vaccines. Researchers are working on overcoming challenges in circRNA synthesis for future applications.
Circular RNA (circRNA) is a unique type of noncoding RNA molecule. Compared with traditional linear RNA, circRNA is a covalently closed circle produced by a process called backsplicing. CircRNA is abundant in many cells and has rich functions in cells, such as acting as miRNA sponge, protein sponge, protein scaffold, and mRNA regulator. With the continuous development of circRNA study, circRNA has also played an important role in medical applications, including circRNA vaccines and gene therapy. In this review, we illustrate the synthesis of circRNAs in vitro. We focus on biological ligation methods, such as enzymatic ligation from the bacteriophage T4 and ribozyme method. In addition, we summarize the current challenges in the design, synthesis, application, and production of circRNAs, and propose possible solutions in the future. CircRNA is expected to play an essential role in basic research and medical applications.
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