期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.820507
关键词
malonic acid; a-keto decarboxylase; oxaloacetic acid; metabolic engineering; Myceliophthora thermophila
资金
- Key Project of the Ministry of Science and Technology of China [2018YFA0900500, 2018YFA0901400]
- Chinese Academy of Sciences [XDA21060900]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2020183]
This study presents a novel biological pathway for producing malonic acid from renewable resources and successfully constructed this synthetic pathway in a cellulolytic thermophilic fungus.
Malonic acid is used as a common component of many products and processes in the pharmaceutical and cosmetic industries. Here, we designed a novel artificial synthetic pathway of malonic acid, in which oxaloacetate, an intermediate of cytoplasmic reductive tricarboxylic acid (rTCA) pathway, is converted to malonic semialdehyde and then to malonic acid, sequentially catalyzed by a-keto decarboxylase and malonic semialdehyde dehydrogenase. After the systematic screening, we discovered the enzyme oxaloacetate decarboxylase Mdc, catalyzing the first step of the artificially designed pathway in vitro. Then, this synthetic pathway was functionally constructed in cellulolytic thermophilic fungus Myceliophthora thermophila. After enhancement of glucose uptake, the titer of malonic acid achieved 42.5 mg/L. This study presents a novel biological pathway for producing malonic acid from renewable resources in the future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据