期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.794818
关键词
Gelidium crinale; sulfated polysaccharide; structural characterization; anti-oxidation; anti-inflammation
资金
- Shenzhen International Scientific and Technological Cooperation RD Project [GJHZ20190823111601682]
- Natural Science Foundation of Guangdong Province [2020A1515011075]
- Development Project about Marine Economy Demonstration of Zhanjiang City [XM-202008-01B1]
- Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) [ZJW-2019-07]
A sulfated polysaccharide (GNP) extracted from Gelidium crinale exhibits strong antioxidant and anti-inflammatory activities, inhibiting inflammatory response induced by LPS and showing potential as a component in pharmaceutical and functional food industries.
Gelidium crinale, the red algae belonging to Geliaceae Gelidium, is a traditional edible and industrial alga in China. A sulfated polysaccharide (GNP) is successfully separated from Gelidium crinale by acid extraction and two-step column chromatography. Chemical analysis showed that the molecular weight of GNP was 25.8 kDa and the monosaccharide composition had the highest galactose content and confirmed the presence and content (16.5%) of sulfate by Fourier transform infrared spectroscopy (FT-IR) spectrometry as well as barium chloride-gelatin methods. In addition, the effect of GNP on lipopolysaccharide (LPS)-induced oxidative stress and inflammation in macrophages was also evaluated. The research results showed that GNP had fairly strong scavenging activities on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and had Fe2+-chelating ability in a dose-dependent manner. At the same time, it significantly inhibits the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the production of proinflammatory cytokines in RAW 264.7 cells induced by LPS through blocking the mitogen-activated protein kinase (MAPK)/nuclear factor kappa beta (NF-kappa B) signaling pathway. These results indicate that GNP may be a latent component anti-inflammation in pharmaceutical and functional food industries.
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