期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.756399
关键词
In vitro tools; drug testing; disease modelling; macromolecular crowding; fibrosis; anti-fibrotic molecules
资金
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme [866126]
- Science Foundation Ireland (SFI) [15/CDA/3629, 19/FFP/6982]
- European Regional Development Fund (ERDF) [13/RC/2073_2]
- Finnish National Agency for Education (EDUFI)
- Paivikki and Sakari Sohlberg Foundation
- Tampere Tuberculosis Foundation
- Tampere University Hospital Research Fund
- Pirkanmaa Hospital District Research Foundation
- Academy of Finland
Skin fibrosis is still an unmet clinical need, and the poor predictive capacity of currently available in vitro fibrosis models hinders their clinical translation. This study utilized macromolecular crowding and TGF beta 1 to develop a skin fibrosis in vitro model and screen a range of anti-fibrotic compounds, demonstrating the potential of macromolecular crowding in the development of in vitro pathophysiology models.
Skin fibrosis still constitutes an unmet clinical need. Although pharmacological strategies are at the forefront of scientific and technological research and innovation, their clinical translation is hindered by the poor predictive capacity of the currently available in vitro fibrosis models. Indeed, customarily utilised in vitro scarring models are conducted in a low extracellular matrix milieu, which constitutes an oxymoron for the in-hand pathophysiology. Herein, we coupled macromolecular crowding (enhances and accelerates extracellular matrix deposition) with transforming growth factor beta 1 (TGF beta 1; induces trans-differentiation of fibroblasts to myofibroblasts) in human dermal fibroblast cultures to develop a skin fibrosis in vitro model and to screen a range of anti-fibrotic families (corticosteroids, inhibitors of histone deacetylases, inhibitors of collagen crosslinking, inhibitors of TGF beta 1 and pleiotropic inhibitors of fibrotic activation). Data obtained demonstrated that macromolecular crowding combined with TGF beta 1 significantly enhanced collagen deposition and myofibroblast transformation. Among the anti-fibrotic compounds assessed, trichostatin A (inhibitors of histone deacetylases); serelaxin and pirfenidone (pleiotropic inhibitors of fibrotic activation); and soluble TGF beta receptor trap (inhibitor of TGF beta signalling) resulted in the highest decrease of collagen type I deposition (even higher than triamcinolone acetonide, the gold standard in clinical practice). This study further advocates the potential of macromolecular crowding in the development of in vitro pathophysiology models.
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