期刊
JCI INSIGHT
卷 6, 期 22, 页码 -出版社
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/jci.insight.152288
关键词
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资金
- Massachusetts Consortium on Pathogen Readiness
- Mathers Foundation
- Department of Defense [W81XWH2110029]
The study found that macrophages and neutrophils are increased in nasal wash cells of patients infected with COVID-19 and influenza, with an enrichment of specific cell types such as hillock-like epithelial cells, M2-like macrophages, and age-dependent B cells in COVID-19 samples. Compared to influenza, a global decrease in IFN-associated transcripts in neutrophils, macrophages, and epithelial cells was observed in COVID-19 samples.
BACKGROUND. Influenza A virus (IAV) and SARS-CoV-2 are pandemic viruses causing millions of deaths, yet their clinical manifestations are distinctly different. METHODS. With the hypothesis that upper airway immune and epithelial cell responses are also distinct, we performed single-cell RNA sequencing (scRNA-Seq) on nasal wash cells freshly collected from adults with either acute COVID-19 or influenza or from healthy controls. We focused on major cell types and subtypes in a subset of donor samples. RESULTS. Nasal wash cells were enriched for macrophages and neutrophils for both individuals with influenza and those with COVID-19 compared with healthy controls. Hillock-like epithelial cells, M2-like macrophages, and age-dependent B cells were enriched in COVID-19 samples. A global decrease in IFN-associated transcripts in neutrophils, macrophages, and epithelial cells was apparent in COVID-19 samples compared with influenza samples. The innate immune response to SARS-CoV-2 appears to be maintained in macrophages, despite evidence for limited epithelial cell immune sensing. Cell-to-cell interaction analyses revealed a decrease in epithelial cell interactions in COVID-19 and highlighted differences in macrophage-macrophage interactions for COVID-19 and influenza. CONCLUSIONS. Our study demonstrates that scRNA-Seq can define host and viral transcriptional activity at the site of infection and reveal distinct local epithelial and immune cell responses for COVID-19 and influenza that may contribute to their divergent disease courses.
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