Identification of organophosphate modifications by high-resolution mass spectrometry

Organophosphate (OP) compounds exhibit neurotoxicity by binding to serine residues of acetylcholinesterase (AChE) in the cholinergic nervous system and subsequently lead to the accumulation of acetylcholine in neuromuscular junctions of synapses. AChE capable of hydrolyzing choline esters is known to be inhibited in patients with nerve agents poisoning. Since OP is known to be transported by covalent bonding to human serum albumin (HSA), OP-HSA adducts are considered potential diagnostic markers for OP exposures. In this study, HSA modification sites by OP or OP-like compounds such as V-type (VX) and Novichok-type (A234), insecticide (DFP), and serine protease inhibitor (PMSF) were studied using liquid chromatography-high-resolution tandem mass spectrometry. As a result, we have discovered a novel OP-HSA modification site, Y341.

Automated summary

Organophosphate compounds exhibit neurotoxicity by binding to serine residues of acetylcholinesterase in the cholinergic nervous system, leading to the accumulation of acetylcholine in synapses. These compounds are transported by covalent bonding to human serum albumin, forming potential diagnostic markers for exposure. The study identified a novel OP-HSA modification site, Y341, using liquid chromatography-high-resolution tandem mass spectrometry.