4.4 Article

Efficacy of chitinase-3-like protein 1 as an in vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells

期刊

REGENERATIVE THERAPY
卷 18, 期 -, 页码 38-50

出版社

ELSEVIER
DOI: 10.1016/j.reth.2021.03.004

关键词

Jaw bone marrow stromal cells; Bone formation capacity; Chitinase-3-like protein 1; Migration

资金

  1. Japan Society for the Promotion of Science [19K10212, 20H03881]
  2. Grants-in-Aid for Scientific Research [19K10212, 20H03881] Funding Source: KAKEN

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This study identified the impact of CHI3L1 secretion levels on the in vivo bone formation capacity of MBMSCs, providing important insights for the clinical application of MBMSCs. It was found that the secretion levels of CHI3L1 can be used as a predictive marker for the bone formation capacity of MBMSCs in vivo.
Introduction: Maxillary/mandibular bone marrow stromal cells (MBMSCs) are a useful cell source for bone regeneration in the oral and maxillofacial region. To further ensure the clinical application of MBMSCs in bone regenerative therapy, it is important to determine the bone formation capacity of MBMSCs before transplantation. The aim of this study is to identify the molecular marker that determines the in vivo bone formation capacity of MBMSCs. Methods: The cell growth, cell surface antigens, in vitro and in vivo bone formation capacity of MBMSCs were examined. The amount of chitinase-3-like protein 1 (CHI3L1) secreted into the conditioned medium was quantified. The effects of CHI3L1 on the cell growth and osteogenic differentiation potential of MBMSCs and on the cell growth and migration of vascular endothelial cells and fibroblasts were examined. Results: The cell growth, and in vitro and in vivo bone formation capacity of the cells treated with different conditions were observed. MBMSCs that secreted a large amount of CHI3L1 into the conditioned medium tended to have low in vivo bone formation capacity, whereas MBMSCs that secreted a small amount of CHI3L1 had greater in vivo bone formation capacity. CHI3L1 promoted the migration of vascular endothelial cells, and the cell growth and migration of fibroblasts. Conclusion: Our study indicates that the in vitro osteogenic differentiation capacity of MBMSCs and the in vivo bone formation capacities of MBMSCs were not necessarily correlated. The transplantation of high CHI3L1 secretory MBMSCs may suppress bone formation by inducing fibrosis at the site. These results suggest that the CHI3L1 secretion levels from MBMSCs may be used as a predictable marker of bone formation capacity in vivo. (C) 2021, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.

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