Fermented Dendrobium officinale polysaccharides protect UVA-induced photoaging of human skin fibroblasts

In this study, Fourier transform infrared spectroscopy (FT-IR), gel permeation chromatograph-liquid chromatography (GPC-LC), and scanning electron microscopy (SEM) were used to analyze the molecular characteristics of fermented Dendrobium officinale polysaccharides (FDOP) by Lactobacillus delbrueckil bulgaricus. The characteristic structural peak of FDOP was more prominent, showing a smaller molecular structure, and its porous structure showed better water solubility. The protective effect of FDOP on the damage of human skin fibroblasts (HSF) caused by ultraviolet (UV) radiation was investigated by evaluating its antioxidative and antiaging indices. The results showed that the antioxidant capacity of HSF was improved, and the breakdown of collagen, elastin, and hyaluronic acid was reduced, thus providing effective protection to the skin tissue. The antioxidative property of FDOP was explored using Nf-E2-related factor 2-small interfering RNA-3 (Nrf2-siRNA-3) (Nrf2-si3) and qRT-PCR (quantitative reverse transcription polymerase chain reaction), and the antiaging property of FDOP was explored using Western Blot and qRT-PCR. The results show that FDOP can up-regulate signal transduction of the Nrf2/Keapl. (Ketch-like ECH-associated protein 1) and transforming growth factor-beta (TGF-beta)/Smads pathways to reduce antioxidative damage and antiaging effects. Therefore, this study provides a theoretical basis for FDOP as a novel functional agent that can be used in the cosmetic industry.

Automated summary

In this study, the molecular characteristics of fermented Dendrobium officinale polysaccharides (FDOP) by Lactobacillus delbrueckil bulgaricus were analyzed using FT-IR, GPC-LC, and SEM. FDOP showed a smaller molecular structure and better water solubility. The protective effect of FDOP on human skin fibroblasts (HSF) damaged by UV radiation was investigated, and it was found that FDOP improved the antioxidant capacity of HSF and reduced the breakdown of collagen, elastin, and hyaluronic acid. The antioxidative and antiaging properties of FDOP were explored, and it was observed that FDOP could up-regulate the Nrf2/Keapl and TGF-beta/Smads pathways to reduce oxidative damage and aging effects.