Molecular Survey of Babesia spp. and Anaplasma phagocytophilum in Roe Deer from a Wildlife Rescue Center in Italy

Simple Summary Roe deer, whose populations are increasing and expanding throughout Europe, are suitable hosts for the maintenance of a variety of infectious tick-vectored microorganisms, that can infect both animals and humans. In this study the presence of tick-transmitted pathogens was investigated in roe deer recovered by a wildlife rescue center based in Italy. This kind of samples represents a convenient material for investigations under several aspects for both animals and researchers. Notably, no live trapping or killing are required to obtain samples, as they are collected in the context of the rescue activities and aimed to frame the health status of the animal. The investigated blood samples showed high positive rates to typical roe deer-related microorganisms (such as Babesia capreoli), and to the zoonotic agent Babesia venatorum. Roe deer were also positive to Anaplasma phagocytophilum strains mainly considered apathogenic or limited to wild ungulates. The obtained results underline the importance of a constant investigation on circulating tick-borne pathogens in roe deer, and generally speaking, in wild animal species, due to their potential role as a key factor in the endemic cycle of important infectious agents for domestic and wild animals, as well as humans. Babesia ssp. and Anaplasma spp. are tick-borne microorganisms representing a possible health risk for domestic and wild animals, as well as humans. Roe deer serve as a suitable reservoir host for some species ascribed to Babesia spp. and Anaplasma phagocytophilum taxa, also due to its important role in the maintenance of large populations of Ixodes ricinus, the main tick vector of these pathogens in Europe. Roe deer populations have been recently expanding throughout Europe, namely in Italy. However, the collection of samples from free-ranging wild animals for diagnostic investigations often includes several practical issues. This problem can be overcome using samples provided by wildlife rescue centers making them available for investigations following routine analyses. The presence of Babesia spp. and Anaplasma spp. in blood samples of 43 roe deer rescued by a wildlife rescue center in Emilia-Romagna region (Italy) was molecularly investigated. PCR screening revealed the presence of at least one pathogen in 86.05% of the animals, while co-infection occurred in 18.92% of the tested individuals. Zoonotic Babesia venatorum was found in 6.98% of the samples, while Babesia capreoli and Anaplasma phagocytophilum were detected in 74.42% and in 20.93%, respectively. No hematological signs compatible with clinical anaplasmosis or piroplasmosis, as well as absence of intracellular circulating microorganisms in blood smears, were observed, suggesting asymptomatic infection in the tested animals. These results confirm the usefulness of wild rescued animals as convenient source of biological samples for tick-borne pathogens investigation and the role of roe deer as a key factor in the endemic cycle of Babesia species and A. phagocytophilum.

Automated summary

This study investigated the presence of tick-transmitted pathogens in roe deer recovered by a wildlife rescue center in Italy, with high positive rates found. These results emphasize the usefulness of wild rescued animals as a convenient source of biological samples for tick-borne pathogens investigation and highlight the role of roe deer in the endemic cycle of Babesia species and Anaplasma phagocytophilum.