Identification of Monoallelically Expressed Genes Associated with Economic Traits in Hanwoo (Korean Native Cattle)

Simple Summary Although most genes are expected to transcribe both alleles evenly in diploid animals, some genes show monoallelic expression (MAE). The expressed allele can be determined randomly or by parental origin (known as genomic imprinting). Here, we conducted genome-wide and transcriptome-wide screening of MAE genes in Korean cattle (Hanwoo) and identified tissue-specific MAE pattens. The effects of MAE genes on phenotypic variation were evaluated by association analysis with the breeding values of five traits that were used in a Hanwoo breeding program. Hanwoo, an indigenous Korean cattle breed, has been genetically improved by selecting superior sires called Korean-proven bulls. However, cows still contribute half of the genetic stock of their offspring, and allelic-specific expressed genes have potential, as selective targets of cows, to enhance genetic gain. The aim of this study is to identify genes that have MAEs based on both the genome and transcriptome and to estimate their effects on breeding values (BVs) for economically important traits in Hanwoo. We generated resequencing data for the parents and RNA-sequencing data for the muscle, fat, and brain tissues of the offspring. A total of 3801 heterozygous single nucleotide polymorphisms (SNPs) in offspring were identified and they were located in 1569 genes. Only 14 genes showed MAE (seven expressing maternal alleles and seven expressing paternal alleles). Tissue-specific MAE was observed, and LANCL1 showed maternal allele expression across all tissues. MAE genes were enriched for the biological process of cell death and angiogenesis, which included ACKR3 and PDCL3 genes, whose SNPs were significantly associated with BVs of lean meat production-related traits, such as weight at 12 months of age, carcass weight, and loin eye area. In the current study, monoallelically expressed genes were identified in various adult tissues and these genes were associated with genetic capacity in Hanwoo.

Automated summary

In this study, genes with monoallelic expression were identified in Korean cattle (Hanwoo) using genome-wide and transcriptome-wide screening. Tissue-specific expression patterns were observed, and these genes were associated with breeding values for economically important traits in Hanwoo. This study provides potential selective targets for genetic improvement in Hanwoo.