期刊
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
卷 20, 期 1, 页码 231-245出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10942912.2016.1154573
关键词
Macaca fascicularis; Short-amplicon-length PCR assay; Mitochondrial d-loop gene; Religious and wildlife restriction; Restriction fingerprints
资金
- University of Malaya fund PPP fund [PG0004-2013B, GC001A-14SBS]
The macaque (Macaca fascicularis) monkeys are the third largest primate population in tropical forests. Being the potential carrier of Simian Immunodeficiency and Ebola and Corona viruses, as well as being a reli-giously-protected and wildlife-protected species could not save the macaques from being over-hunted and consumed. Despite having needs, methods to detect monkey species in foods are rarely documented. To fill this gap, this article describes a monkey-specific polymerase chain reaction assay targeting a short-site (120 bp) of mitochondrial d-loop gene because the short-length targets are thermodynamically more stable than the longer ones under degrading states. Specificity was tested against 17 terrestrial and aquatic meat and fish species and no cross-species amplification was detected under raw, processed, and admixed states. The sensitivity of the assay was 0.0001 ng DNA under pure states and 0.1% monkey meats in binary meat mixtures. Finally, the assay was validated by digesting the polymerase chain reaction products with AluI and CViKI-1 and distinctive restriction fingerprints for macaque species were demonstrated.
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