期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 239, 期 -, 页码 95-102出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2016.06.010
关键词
Sourdough; Reuteransucrase; Bread quality; Reuteran; Dextran; Exopolysaccharides
Exopolysaccharides from lactic acid bacteria may improve texture and shelf life of bread. The effect of exopolysaccharides on bread quality, however, depends on properties of the EPS and the EPS producing strain. This study investigated structure-function relationships of EPS in baking application. The dextransucrase DsrM and the reuteransucrase GtfA were cloned from Weissella cibaria 10M and Lactobacillus reuteri TMW1.656, respectively, and heterologously expressed in Escherichia coli. Site-directed mutagenesis of GtfA was generates reuterans with different glycosidic bonds. NMR spectrum indicated reuteranPl, reuteranNS and reuteranPlNS produced by GtfA-V1024P:V10271, GtfA-S1135N:A1137S and GtfA-V1024P:V1027l:S1135N:A11375, respectively, had a higher proportion of alpha-(1 -> 4) linkages when compared to reuteran. ReuteranNS has the lowest molecular weight as measured by asymmetric flow-field-flow fractionation. The reuteransucrase negative mutant L reuteriTMW1.6564 Delta gtfA was generated as EPS-negative derivative of L. reuteriTMW1.656. Cell counts, pH, and organic acid levels of sourdough fermented with L. reuteri TMW1.656 and TMW1.656 Delta gtfA were comparable. Reuteran produced by L. reuteri TMW1.656 during growth in sourdough and reuteran produced ex situ by GtfA-Delta N had comparable effects on bread volume and crumb hardness. Enzymatically produced dextran improved volume and texture of wheat bread, and of bread containing 20% rye flour. ReuteranNS but not reuteranPl or reuteran was as efficient as dextran in enhancing wheat bread volume and texture. Overall, reuteran linkage type and molecular weight are determinants of EPS effects on bread quality. This study established a valuable method to elucidate structure-function relationships of glucans in baking applications. (C) 2016 Elsevier B.V. All rights reserved.
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