期刊
WORLD JOURNAL OF MENS HEALTH
卷 40, 期 4, 页码 627-635出版社
KOREAN SOC SEXUAL MEDICINE & ANDROLOGY
DOI: 10.5534/wjmh.210210
关键词
Azoospermia; Centrifugation; Male infertility; Semen; Staining and labeling
资金
- American Center for Reproductive Medicine
Cytospin centrifugation combined with nuclear fast picroindigocarmine staining can identify sperm in azoospermic samples and change the diagnosis and treatment for these patients.
Purpose: Azoospermia is defined as the absence of spermatozoa in the pellet of a centrifuged semen sample. In fact, when a basic semen analysis fails to detect sperm in the ejaculate, there is still the possibility of detecting rare sperm after centrifugation of the sample and examination of the pellet. In this study, we assessed the role of Cytospin centrifugation in combination with the nuclear fast picroindigocarmine (NF-PIC) staining in identifying sperm in azoospermic samples. Materials and Methods: Semen samples of 251 men diagnosed as having azoospermia after standard examination were further analyzed by Cytospin centrifugation in combination with NF-PIC staining. Results: Sperm were detected in 60 men (23.9%), thus changing their diagnosis to cryptozoospermia. Conclusions: By identifying sperm in the semen of men who were thought to have total azoospermia, the Cytospin NF-PIC test can alter the diagnosis and further treatment of these men.
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