期刊
PHARMACEUTICS
卷 14, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/pharmaceutics14010140
关键词
arthritis; flurbiprofen; nanoparticles; intra-articular; hyaluronic acid; active targeting
This study formulated hyaluronic acid-coated bovine serum albumin nanoparticles loaded with flurbiprofen for the treatment of arthritis. The nanoparticles exhibited good physicochemical properties and demonstrated a promising therapeutic effect in both in-vitro and in-vivo studies.
Flurbiprofen (FLUR) is a potent non-steroidal anti-inflammatory drug used for the management of arthritis. Unfortunately, its therapeutic effect is limited by its rapid clearance from the joints following intra-articular injection. To improve its therapeutic efficacy, hyaluronic acid-coated bovine serum albumin nanoparticles (HA-BSA NPs) were formulated and loaded with FLUR to achieve active drug targeting. NPs were prepared by a modified nano-emulsification technique and their HA coating was proven via turbidimetric assay. Physicochemical characterization of the selected HA-BSA NPs revealed entrapment efficiency of 90.12 +/- 1.06%, particle size of 257.12 +/- 2.54 nm, PDI of 0.25 +/- 0.01, and zeta potential of -48 +/- 3 mv. The selected formulation showed in-vitro extended-release profile up to 6 days. In-vivo studies on adjuvant-induced arthritis rat model exhibited a significant reduction in joint swelling after intra-articular administration of FLUR-loaded HA-BSA NPs. Additionally, there was a significant reduction in CRP level in blood as well as TNF-alpha, and IL-6 levels in serum and joint tissues. Immunohistochemical study indicated a significant decrease in iNOS level in joint tissues. Histopathological analysis confirmed the safety of FLUR-loaded HA-BSA NPs. Thus, our results reveal that FLUR loaded HA-BSA NPs have a promising therapeutic effect in the management of arthritis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据