期刊
PHARMACEUTICS
卷 13, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/pharmaceutics13101564
关键词
oral bacteria; Rothia mucilaginosa; TiO(2)NPs; biofilm inhibition
资金
- Priority Research Centers Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2014R1A6A1031189, RSP-2021/31]
- King Saud University, Riyadh, Saudi Arabia
The study isolated and identified three Gram-positive MDR oral cocci, characterized their biofilm forming ability, and found that TiO(2)NPs have inhibitory effects on biofilm formation.
Multi-drug resistant (MDR) bacterial cells embedded in biofilm matrices can lead to the development of chronic cariogenesis. Here, we isolated and identified three Gram-positive MDR oral cocci, (1) SJM-04, (2) SJM-38, and (3) SJM-65, and characterized them morphologically, biochemically, and by 16S rRNA gene-based phylogenetic analysis as Georgenia sp., Staphylococcus saprophyticus, and Rothia mucilaginosa, respectively. These three oral isolates exhibited antibiotic-resistance against nalidixic acid, tetracycline, cefuroxime, methicillin, and ceftazidime. Furthermore, these Gram positive MDR oral cocci showed significant (p < 0.05) variations in their biofilm forming ability under different physicochemical conditions, that is, at temperatures of 28, 30, and 42 & DEG;C, pH of 6.4, 7.4, and 8.4, and NaCl concentrations from 200 to 1000 mu g/mL. Exposure of oral isolates to TiO(2)NPs (14.7 nm) significantly (p < 0.05) reduced planktonic cell viability and biofilm formation in a concentration-dependent manner, which was confirmed by observing biofilm architecture by scanning electron microscopy (SEM) and optical microscopy. Overall, these results have important implications for the use of tetragonal anatase phase TiO(2)NPs (size range 5-25 nm, crystalline size 13.7 nm, and spherical shape) as an oral antibiofilm agent against Gram positive cocci infections. We suggest that TiO(2)NPs pave the way for further applications in oral mouthwash formulations and antibiofilm dental coatings.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据