期刊
ADVANCED MATERIALS TECHNOLOGIES
卷 7, 期 1, 页码 -出版社
WILEY
DOI: 10.1002/admt.202100836
关键词
cell culture; microcarrier; microfluidic; particle; poly(3,4-ethylenedioxythiophene):polystyrene sulfonate
资金
- DFG [WE 4678/12-1]
- European Research Council under the European Union's Horizon 2020 research and innovation program [694946]
- EU
- federal state of North Rhine-Westphalia [EFRE 30 00 883 02]
- Projekt DEAL
This study presents a novel microfluidic process for the synthesis of pure PEDOT:PSS particles with adjustable properties. The particles can be tailored in terms of crystallinity, diameter, pore size, and electrochemical/mechanical properties by adjusting the IPA:SA ratio during synthesis. Experiments demonstrate the potential of PEDOT:PSS particles as a cell culture substrate for both mouse fibroblast cells and human fibroblast cells, showing exponential growth and high cell viability.
Due to its biocompatibility, electrical conductivity, and tissue-like elasticity, poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) constitutes a highly promising material regarding the fabrication of smart cell culture substrates. However, until now, high-throughput synthesis of pure PEDOT:PSS geometries was restricted to flat sheets and fibers. In this publication, the first microfluidic process for the synthesis of spherical, highly porous, pure PEDOT:PSS particles of adjustable material properties is presented. The particles are synthesized by the generation of PEDOT:PSS emulsion droplets within a 1-octanol continuous phase and their subsequent coagulation and partial crystallization in an isopropanol (IPA)/sulfuric acid (SA) bath. The process allows to tailor central particle characteristics such as crystallinity, particle diameter, pore size as well as electrochemical and mechanical properties by simply adjusting the IPA:SA ratio during droplet coagulation. To demonstrate the applicability of PEDOT:PSS particles as potential cell culture substrate, cultivations of L929 mouse fibroblast cells and MRC-5 human fibroblast cells are conducted. Both cell lines present exponential growth on PEDOT:PSS particles and reach confluency with cell viabilities above 95 vol.% on culture day 9. Single cell analysis could moreover reveal that mechanotransduction and cell infiltration can be controlled by the adjustment of particle crystallinity.
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