期刊
CELLS
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/cells11030555
关键词
CDK5RAP3; ER stress; gamma-tubulin; microtubule nucleation; UFL1
类别
资金
- Czech Science Foundation [18-27197S, 19-20716S, 21-30281S]
- Ministry of Education, Youth and Sports of the Czech Republic (MEYS) [LTAUSA17052, LTAUSA19118]
- Technology Agency of the Czech Republic [TP01010060]
- Institutional Research Support (RVO) [68378050]
- MEYS [LM2018129]
ER distribution relies on microtubules, and disruption of ER homeostasis activates the unfolded protein response, leading to ER remodeling. UFL1 and C53 interact with gamma-tubulin ring complex proteins and regulate microtubule nucleation in response to ER stress. These findings reveal a novel mechanism for alleviating ER stress through stimulation of centrosomal microtubule nucleation.
ER distribution depends on microtubules, and ER homeostasis disturbance activates the unfolded protein response resulting in ER remodeling. CDK5RAP3 (C53) implicated in various signaling pathways interacts with UFM1-protein ligase 1 (UFL1), which mediates the ufmylation of proteins in response to ER stress. Here we find that UFL1 and C53 associate with gamma-tubulin ring complex proteins. Knockout of UFL1 or C53 in human osteosarcoma cells induces ER stress and boosts centrosomal microtubule nucleation accompanied by gamma-tubulin accumulation, microtubule formation, and ER expansion. C53, which is stabilized by UFL1, associates with the centrosome and rescues microtubule nucleation in cells lacking UFL1. Pharmacological induction of ER stress by tunicamycin also leads to increased microtubule nucleation and ER expansion. Furthermore, tunicamycin suppresses the association of C53 with the centrosome. These findings point to a novel mechanism for the relief of ER stress by stimulation of centrosomal microtubule nucleation.
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