4.6 Article

TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression

期刊

CELLS
卷 10, 期 10, 页码 -

出版社

MDPI
DOI: 10.3390/cells10102751

关键词

TWIK-1; bacterial artificial chromosome transgenic mouse; dentate gyrus; lateral entorhinal cortex; cerebellum

资金

  1. National Research Foundation (NRF) of Korea [NRF-2017R1A2B3012502, NRF-2017M3C7A1046154, NRF-2019R1A6A3A13096591]
  2. National Research Foundation of Korea [2017M3C7A1046154] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

向作者/读者索取更多资源

TWIK-1 BAC-GFP Tg mice expressing GFP under TWIK-1 promoter control show high GFP expression in various brain areas, including the DG, LEC, and Cb, with particularly high expression in immature granule cells in the DG. These mice also mimic the upregulation of TWIK-1 mRNA expression in the hippocampus following KA injection, providing a useful animal model for studying TWIK-1 gene expression regulation and its physiological roles in the brain.
TWIK-1 is the first identified member of the two-pore domain potassium (K2P) channels that are involved in neuronal excitability and astrocytic passive conductance in the brain. Despite the physiological roles of TWIK-1, there is still a lack of information on the basic expression patterns of TWIK-1 proteins in the brain. Here, using a modified bacterial artificial chromosome (BAC), we generated a transgenic mouse (Tg mouse) line expressing green fluorescent protein (GFP) under the control of the TWIK-1 promoter (TWIK-1 BAC-GFP Tg mice). We confirmed that nearly all GFP-producing cells co-expressed endogenous TWIK-1 in the brain of TWIK-1 BAC-GFP Tg mice. GFP signals were highly expressed in various brain areas, including the dentate gyrus (DG), lateral entorhinal cortex (LEC), and cerebellum (Cb). In addition, we found that GFP signals were highly expressed in immature granule cells in the DG. Finally, our TWIK-1 BAC-GFP Tg mice mimic the upregulation of TWIK-1 mRNA expression in the hippocampus following the injection of kainic acid (KA). Our data clearly showed that TWIK-1 BAC-GFP Tg mice are a useful animal model for studying the mechanisms regulating TWIK-1 gene expression and the physiological roles of TWIK-1 channels in the brain.

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