期刊
CELLS
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/cells10113051
关键词
skin; dermis; pericyte; microenvironment; human; ageing
类别
资金
- National Health & Medical Research Council of Australia [1043453]
- Australian Postgraduate Award
Skin ageing is a complex process influenced by various factors, with a decline in the number and function of pericytes potentially playing a significant role in impaired skin regeneration.
The well documented decline in the regenerative ability of ageing human skin has been attributed to many factors including genomic instability, telomere shortening, poor nutrient sensing, cellular senescence, and stem cell exhaustion. However, a role for the dermal cellular and molecular microenvironment in skin ageing is just emerging. We previously showed that dermal pericytes co-operate with fibroblasts to improve human skin regeneration in an organotypic skin culture model, and even do so in the absence of fibroblasts. Here, we report that the number of dermal cells, particularly pericytes, declines significantly in human skin of donors aged > 50 years. Notably, aged pericytes promoted epidermal regeneration of neonatal keratinocytes in organotypic cultures and the resulting epithelium exhibited a Ki67(+)/& UDelta;Np63(+) basal layer and terminal differentiation. However, the epithelium lacked several features of homeostasis displaying lower levels of & UDelta;Np63 expression, decreased LAMA5 deposition at the dermo-epidermal junction, and the absence of basement membrane and hemi-desmosome assembly. We conclude that a decline in pericyte incidence and function contribute to an impaired epidermal microenvironment and poor skin regeneration with ageing in the human skin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据