期刊
CELLS
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/cells11030511
关键词
colorectal cancer; CRC; cancer stem cells; cancer-initiating cells; oxaliplatin; drug resistance; tumor recurrence; chemotherapy
类别
资金
- Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Spain [PI20-00335, PI20-00329]
- European Regional Development (FEDER) funds
This article explores the relationship between colorectal cancer stem cells and oxaliplatin-resistant cells. The results show that cancer stem cells have increased resistance to the chemotherapy drug oxaliplatin, and resistant cells contain more cancer stem cells. Additionally, both cell types exhibit increased cell invasion capacity and epithelial-mesenchymal transition. mRNA expression analysis reveals a significant proportion of commonly regulated genes in these two cell types.
Colorectal cancer is the second most common cancer in women, the third in men, and an important cause of cancer-related mortality. Recurrence and the development of chemotherapy resistance are major hindrances for patients' treatment. The presence of cancer stem cells with chemotherapy resistance able to generate proliferating tumor cells contributes to tumor recurrence and resistance. In addition, tumor cells can develop chemoresistance through adaptation mechanisms. In this article, cancer stem cells were isolated from HT29 and SW620 colorectal cancer cell lines. Oxaliplatin resistance was induced by a single drug treatment simulating the usual guidelines of patient treatment. A comparison of these two populations showed similarities since cancer stem cells presented increased oxaliplatin resistance, and resistant cells contained an increased number of cancer stem cells. Cancer stem cells isolated from resistant cells showed increased oxaliplatin resistance. Cell invasion capacity and epithelial-mesenchymal transition were increased both in cancer stem cells and oxaliplatin-resistant cells. mRNA expression analysis showed that both cell types shared a significant proportion of commonly regulated genes. In summary, the data presented indicate that colorectal cancer stem cells and oxaliplatin-resistant cells are highly related cell populations that might have interesting implications in the development of tumor recurrence and resistance to chemotherapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据