期刊
CELLS
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/cells10113240
关键词
serotonin; Tph2; depression; neurogenesis; stem cells; BrdU; behavior
类别
资金
- Rahel Hirsch Fellowship, Charite University Medicine Berlin
- Russian Foundation for Basic Research (RFBR) [20-015-00470]
- St. Petersburg State University [73022475]
The study utilized a new transgenic rat model to investigate the effects of changes in 5-HT levels, and found that alterations in 5-HT concentrations in different brain regions can trigger diverse physiological and behavioral responses, which may represent a compensatory mechanism.
Serotonin (5-hydroxytryptamine, 5-HT) is a crucial signal in the neurogenic niche of the hippocampus, where it is involved in antidepressant action. Here, we utilized a new transgenic rat model (TetO-shTPH2), where brain 5-HT levels can be acutely altered based on doxycycline (Dox)-inducible shRNA-expression. On/off stimulations of 5-HT concentrations might uniquely mirror the clinical course of major depression (e.g., relapse after discontinuation of antidepressants) in humans. Specifically, we measured 5-HT levels, and 5-HT metabolite 5-HIAA, in various brain areas following acute tryptophan hydroxylase 2 (Tph2) knockdown, and replenishment, and examined behavior and proliferation and survival of newly generated cells in the dentate gyrus. We found that decreased 5-HT levels in the prefrontal cortex and raphe nuclei, but not in the hippocampus of TetO-shTPH2 rats, lead to an enduring anxious phenotype. Surprisingly, the reduction in 5-HT synthesis is associated with increased numbers of BrdU-labeled cells in the dentate gyrus. At 3 weeks of Tph2 replenishment, 5-HT levels return to baseline and survival of newly generated cells is unaffected. We speculate that the acutely induced decrease in 5-HT concentrations and increased neurogenesis might represent a compensatory mechanism.
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