4.6 Article

A DOCK1 Gene-Derived Circular RNA Is Highly Expressed in Luminal Mammary Tumours and Is Involved in the Epithelial Differentiation, Growth, and Motility of Breast Cancer Cells

期刊

CANCERS
卷 13, 期 21, 页码 -

出版社

MDPI
DOI: 10.3390/cancers13215325

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circular RNAs; breast cancer; RNA sequencing; differentiation; epithelial-to-mesenchymal transition (EMT)

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  1. Associazione Italiana per la Ricerca contro il Cancro (AIRC)
  2. Fondazione Italo Monzino
  3. National Breast Cancer Foundation

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Circular RNAs are important regulatory molecules in cellular processes and may be involved in tumor growth and diffusion. The circular RNA from the DOCK1 gene shows differential expression in breast cancer cell lines with different phenotypes and influences the expression of common genes. The study provides insights into the role of specific circular RNAs in the generation and progression of breast cancer.
Circular RNAs are regulatory molecules involved in numerous cellular processes and may be involved in tumour growth and diffusion. Here, we define the expression of 15 selected circular RNAs, which may control the process of epithelial-to-mesenchymal transition, using a panel of 18 breast cancer cell lines recapitulating the heterogeneity of these tumours and consisting of three groups according to the mesenchymal/epithelial phenotype. A circular RNA from the DOCK1 gene (hsa_circ_0020397) shows low/undetectable levels in triple-negative mesenchymal cell lines, while its content is high in epithelial cell lines, independent of estrogen receptor or HER2 positivity. RNA-sequencing experiments performed on the triple-negative/mesenchymal MDA-MB-231 and MDA-MB-157 cell lines engineered to overexpress hsa_circ_0020397 demonstrate that the circRNA influences the expression of 110 common genes. Pathway analysis of these genes indicates that overexpression of the circular RNA differentiates the two mesenchymal cell lines along the epithelial pathway and increases cell-to-cell adhesion. This is accompanied by growth inhibition and a reduction in the random/directional motility of the cell lines. The upregulated AGR2, ENPP1, and PPP1R9A genes as well as the downregulated APOE, AQP3, CD99L2, and IGFBP4 genes show an opposite regulation by hsa_circ_0020397 silencing in luminal CAMA1 cells. The results provide novel insights into the role played by specific circular RNAs in the generation/progression of breast cancer.

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