期刊
SCIENCE ADVANCES
卷 7, 期 44, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abi7785
关键词
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资金
- Spanish Ministry of Science, Education, and Universities [RTI2018-099718-B-100, PGC2018-095663-B100]
- FEDER funds
- ICREA Academia award
Mechanical forces play a role in cytokinesis by activating the mechanosensitive Piezo1 channel at the intercellular bridge; inhibition of Piezo1 leads to multinucleation. Piezo1 generates Ca2+ signals to facilitate ALIX delivery to the intercellular bridge.
Mechanical forces are exerted throughout cytokinesis, the final step of cell division. Yet, how forces are transduced and affect the signaling dynamics of cytokinetic proteins remains poorly characterized. We now show that the mechanosensitive Piezo1 channel is activated at the intercellular bridge (ICB) connecting daughter cells to regulate abscission. Inhibition of Piezo1 caused multinucleation both in vitro and in vivo. Piezo1 positioning at the ICB during cytokinesis depends on Pacsin3. Pharmacological and genetic inhibition of Piezo1 or Pacsin3 resulted in mislocation of Rab11-family-interacting protein 3 (Rab11-FIP3) endosomes, apoptosis-linked gene 2-interacting protein X (ALIX), and endosomal sorting complex required for transport III (ESCRT-III). Furthermore, we identified FIP3 as the link between Piezo1-generated Ca2+ signals and ALIX delivery to the ICB, where ALIX recruits the ESCRT-III component charged multivesicular body protein 4B, which promotes abscission. These results provide a different view of how mechanical forces participate in cytokinesis and identify Piezo1 as a key modulator of endosome trafficking.
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