期刊
SCIENCE ADVANCES
卷 7, 期 51, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abi8307
关键词
-
资金
- Max Planck Society, Germany
- Centre National de la Recherche Scientifique, France
- Sorbonne Universite, France
- German Research Foundation [TRR 175/D02, FOR 804, BO 1482/15-2, FOR 2092, BO 1482/17-1, TI 605/5-1]
- French National Research Agency grant LabEx Dynamo [ANR-LABX-011]
- European Union [862201]
Photosynthetically produced electrons are used to activate Calvin-Benson cycle enzymes and other plastid proteins by reducing specific cysteines via thioredoxins. The redox network in chloroplasts connects the photosynthetic chain with cellular metabolism, allowing fine-tuning of each redox-regulated step. New redox-regulated proteins, involved in Mg2+ transport and signaling, were identified, providing a system-level functional view of the photosynthetic redox regulation network.
Photosynthetically produced electrons provide energy for various metabolic pathways, including carbon reduction. Four Calvin-Benson cycle enzymes and several other plastid proteins are activated in the light by reduction of specific cysteines via thioredoxins, a family of electron transporters operating in redox regulation networks. How does this network link the photosynthetic chain with cellular metabolism? Using a time-resolved redox proteomic method, we have investigated the redox network in vivo during the dark-to-low light transition. We show that redox states of some thioredoxins follow the photosynthetic linear electron transport rate. While some redox targets have kinetics compatible with an equilibrium with one thioredoxin (TRXf), reduction of other proteins shows specific kinetic limitations, allowing fine-tuning of each redox- regulated step of chloroplast metabolism. We identified five new redox-regulated proteins, including proteins involved in Mg2+ transport and O-1(2) signaling. Our results provide a system-level functional view of the photosynthetic redox regulation network.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据