4.6 Article

A Dual-Control Strategy by Phosphate Ions and Local Microviscosity for Tracking Adenosine Triphosphate Metabolism in Mitochondria and Cellular Activity Dynamically

期刊

ACS SENSORS
卷 6, 期 11, 页码 4225-4233

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acssensors.1c01850

关键词

phosphate ion; local microviscosity; fluorescent receptor; mitochondrial targeting; ATP metabolism

资金

  1. National Natural Science Foundation of China [21904077, 22004078, 22074074]
  2. Natural Science Foundation of Shandong Province [ZR2019BB055]

向作者/读者索取更多资源

The sensitive receptor Mito-VP, utilizing the TICT framework, targets mitochondrial ATP metabolism by monitoring Pi levels, providing an efficient tool for tracking mitochondrial health. Responsive fluorescence illumination and capturing of the two steps of ATP hydrolysis help understand the role of energy metabolism at the cellular and organism levels.
Adenosine triphosphate (ATP) acts as the main energy source for growth and development in organisms, and the disorder reflects the mitochondrial damage to a large extent. Therefore, an efficient tool for the evaluation of the ATP metabolic level is important to track mitochondrial health, providing an additional perspective for an in-depth long-term study on living activities. Herein, a twisted intramolecular charge transfer (TICT) framework is utilized to build up a sensitive receptor, Mito-VP, with a negligible background to target mitochondrial ATP metabolism by monitoring the phosphate ion (Pi) level upon ATP hydrolysis under the overall consideration of the structural and functional features of mitochondria. The responsive fluorescence could be lighted on under the dual control of Pi and local microviscosity, and the two steps of ATP hydrolysis could be captured through fluorescence. In addition to the well-behaved mitochondrial targeting, the energy metabolism at cellular and organism levels has been clarified via mitosis and zebrafish development, respectively.

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