期刊
FOOD BIOSCIENCE
卷 43, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fbio.2021.101246
关键词
Flammulina velutipes mycorrhizae; Dietary fiber; Obese mice; Gut microbiota; AMPK signaling Pathway
资金
- National Natural Science Foundation of China [32002329]
- Natural Science Foundation of Shandong Province [ZR2020QC197]
Research shows that Flammulina velutipes dietary fiber can alleviate lipid metabolism disorders in obese mice by regulating the gut microbiota and activating the AMPK signaling pathway, leading to decreased lipid synthesis and increased lipid degradation.
Mounting evidence shows that dietary fiber (DF) alleviates lipid metabolism disorders in humans and animals by regulating the gut microbiota. However, its action mechanisms remain to be elucidated. Flammulina velutipes (F. velutipes) as an edible mushroom is widely cultivated in China and has medicinal values. As a by-product of F. velutipes, the mycorrhizae are rich in high-quality DF and therefore can be used to prepare F. velutipes DF (Fv-DF). The objective of this research aimed to explore whether and how Fv-DF attenuates lipid metabolism disorders in obese mice through the gut microbiota. Here, an obese mice model induced by high-fat diet was established to analyze the effect of Fv-DF supplementation on the lipid metabolism disorders and gut microbiota. The results showed that Fv-DF administration significantly reduced the weight gain, triglycerides and total cholesterol, low-density lipoprotein cholesterol and markedly increased the activity of enzymes related to scavenging ability of oxygen free radicals. The gut microbiota sequencing exhibited that the Fv-DF supplementation increased the level of gut beneficial bacteria, such as Prevotellaceae UCG-001 and Alloprevotella. Western blot analysis revealed the phosphorylated adenosine 5'-monophosphate (AMP)-activated protein kinase (p-AMPK alpha) was up-regulated, indicating that AMPK signaling pathway was activated. The activated pathway down-regulated the fatty acid synthase level to decrease lipid synthesis and up-regulated the carnitine palmitoyl transferase-1 level to increase lipid degradation. Altogether, Fv-DF supplementation alleviates lipid metabolism disorders in obese mice by regulating the gut microbiota-mediated AMPK signaling pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据