Green nanofiber mat from HLM-PVA-Pectin (Hibiscus leaves mucilage-polyvinyl alcohol-pectin) polymeric blend using electrospinning technique as a novel material in wound-healing process

The present work is focused on fabrication of novel nanofiber (NF) mat as wound-healing scaffold using blends of novel combination of Hibiscus rosa-sinensis leaves mucilage (HLM)-Polyvinyl alcohol (PVA)-Pectin, which was never reported previously. Different ratios of the polymeric blends were electrospun by setting different parameters to achieve best possible electrospun nanofiber mat which was later crosslinked by glutaraldehyde vapor. The optimized formulation of nanofiber mat was characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The crosslinked sample was evaluated for its efficacy in wound healing using Swiss albino mice model, where rapid healing of excised wound was observed with faster epithelization in test mice group than control mice within a period of 8 days. The hemolysis test with optimized crosslinked nanofiber mat CrNF(S7-CL) indicated it to be hemo-compatible. There were no traces of optimized CrNF(S7-CL) when placed under the skin hypodermis in test mice groups revealing its biodegradable nature. The degradation pattern of CrNF(S7-CL) in soil reflects its eco-friendly behavior. Thus, the prepared nanofiber grade CrNF(S7-CL) can be considered as a novel material for faster wound healing and can also be explored for other biomedical applications.

Automated summary

The present study focuses on the fabrication of a novel nanofiber mat for wound-healing scaffold using a blend of Hibiscus rosa-sinensis leaves mucilage, polyvinyl alcohol, and pectin. The electrospun nanofiber mat was crosslinked and characterized, and its efficacy in wound healing was evaluated in a mouse model. The optimized crosslinked nanofiber mat showed rapid healing of wounds and biocompatibility, making it a potential material for wound healing and other biomedical applications.