The Apparent Respiratory Quotient of Soils and Tree Stems and the Processes That Control It

The CO2/O-2 fluxes ratio (apparent respiration quotient [ARQ]) measured in soils and plants contains valuable information about the respiratory-substrate stoichiometry and biotic and abiotic non-respiratory processes. We investigated ARQ variability by measurements in soil pore space air, and in headspace air from incubations of bulk-soil and tree stem tissues (both fresh and 24-hr stored tissues) in 10 measurement campaigns over 15 months in a Mediterranean oak forest. Mean (range) ARQ values were: soil air, 0.76 (0.60-0.92); bulk soil, 0.75 (0.53-0.90); fresh stem tissues, 0.39 (0.19-0.70); and stored stem tissues, 0.68 (0.42-1.08). The variability in tree stems was assumed to be controlled by CO2 re-fixation that lowered ARQ from 1.0, the value expected for carbohydrate respiration in plants. We estimate that the values of the stored tissues represent better stem metabolism since the fresh-tissue results contained a signal of wound-response O-2 uptake that further lowered ARQ. The mean bulk-soil ARQ (0.75) was considerably lower than expected by soil organic matter (SOM) stoichiometry (0.95). This lower value might represent the stoichiometry of the SOM sub-pool that supports respiration, and/or oxidative depolymerization that increases O-2 fluxes. Abiotic O-2 uptake was demonstrated to reduce bulk-soil ARQ down to 0.37 and consume Fe2+, but estimated to have small effect under typical respiration rates. Soil-air ARQ was usually higher than bulk-soil ARQ and lower than root ARQ (which, when measured, ranged from 0.73 to 0.96), demonstrating the potential of ARQ to partition the autotrophic and heterotrophic sources of soil respiration. The limitations of this partitioning method are discussed.

Automated summary

The CO2/O2 fluxes ratio, known as the apparent respiration quotient (ARQ), provides valuable information about respiratory-substrate stoichiometry and non-respiratory processes. This study investigated the variability of ARQ in a Mediterranean oak forest over 15 months. The results showed that ARQ values varied in different tissues, with CO2 re-fixation affecting tree stems and wound-response O2 uptake affecting fresh tissues. The study also found that soil ARQ was lower than expected, indicating the stoichiometry of the organic matter sub-pool supporting respiration. The ARQ values in soil air and root air demonstrated the potential to distinguish autotrophic and heterotrophic sources of soil respiration. The limitations of this method were also discussed.