期刊
FRONTIERS IN ENDOCRINOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fendo.2021.793821
关键词
preimplantation genetic testing; non-invasive; blastocyst; spent culture medium; beta-thalassemia
资金
- Health Science and technology project of Guangzhou [20201A011029, 20211A011026]
- Youth medicine research and development program for Reproductive Medicine from Chinese Medical Association [17020150684]
This study compared the detection rates of beta-thalassemia using spent culture medium and spent culture medium containing blastocoelic fluid. The results showed that using spent culture medium containing blastocoelic fluid had a higher detection rate, and the DNA concentration in the spent culture medium was associated with fragmentation.
Objectives: To compare successful beta-thalassemia (beta-thalassemia) detection rates obtained using spent culture medium and spent culture medium containing blastocoelic fluid (BF).Method: This study involved data from 10 couples who underwent preimplantation genetic testing (PGT) for beta-thalassemia. A total of 26 samples of spent culture medium containing BF (group A) and 33 samples without BF (group B) were collected and analyzed. The DNA concentration and beta-thalassemia detection rates were evaluated.Results: The HBB mutation analysis results of 34 samples were concordant with the biopsy results (34/59, 57.6%). In group A, the HBB mutation analysis results of 19 of 26 samples (73.1%) were concordant with the biopsy results. The concordance rate in group A was higher than that in group B (15/33, 45.5%; P < 0.05). The haplotyping results of 38 samples were concordant with the biopsy results (38/59, 64.4%). The concordance rate in group B was 17/33 (51.5%), which was significantly lower than that in group A (21/26, 80.8%) (P < 0.05). In group A, the mean DNA concentration of samples with < 10% fragmentation was 107.3 & PLUSMN; 70.1 ng/mu L, which was lower than that of samples with & GE;10% fragmentation (194.6 & PLUSMN; 28.0 ng/mu L) (P < 0.05). However, the detection rates of < 10% and & GE;10% fragmentation were not significantly different (P > 0.05).Conclusion: The beta-thalassemia detection rate with non-invasive PGT using the spent culture medium containing BF was higher than that using the spent culture medium alone. Fragmentation is associated with DNA concentration in the spent culture medium containing BF.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据