期刊
FRONTIERS IN ENDOCRINOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fendo.2021.816813
关键词
Macrobrachium nipponense; cathepsin L; ovary maturation; mRNA expression; RNA interference
资金
- Jiangsu Agricultural Industry Technology System [JATS [2021] 515]
- National Key R&D Program of China [2018YFD0901303]
- Central Public-interest Scientific Institution Basal Research Fund CAFS [2020TD36]
- China Agriculture Research System-48 [CARS-48]
- New cultivar breeding Major Project of Jiangsu province [PZCZ201745]
In this study, the characteristics and role of cathepsin L in female oriental river prawn were investigated. The results suggest that Mn-CL2 plays a key role in promoting ovary maturation.
Cathepsin L genes, which belonged to cysteine proteases, were a series of multifunctional protease and played important roles in a lot of pathological and physiological processes. In this study, we analyzed the characteristics a cathepsin L (named Mn-CL2) in the female oriental river prawn, Macrobrachium nipponense which was involved in ovary maturation. The Mn-CL2 was1,582 bp in length, including a 978 bp open reading frame that encoded 326 amino acids. The Mn-CL2 was classified into the cathepsin L group by phylogenetic analysis. Real-time PCR (qPCR) analysis indicated that Mn-CL2 was highly expressed in the hepatopancreas and ovaries of female prawns. During the different ovarian stages, Mn-CL2 expression in the hepatopancreas and ovaries peaked before ovarian maturation. In situ hybridization studies revealed that Mn-CL2 was localized in the oocyte of the ovary. Injection of Mn-CL2 dsRNA significantly reduced the expression of vitellogenin. Changes in the gonad somatic index also confirmed the inhibitory effects of Mn-CL2 dsRNA on ovary maturation. These results suggest that Mn-CL2 has a key role in promoting ovary maturation.
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