期刊
MATERIALS & DESIGN
卷 215, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.matdes.2022.110469
关键词
Bone defect; RADA16/CaSO4/HA; Controlled release; Osteogenic differentiation; Bone formation
资金
- Foundation of The First Affiliated Hospital of Chongqing Medical University [PYJJ2018-13]
- Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0836]
- Chongqing Yuzhong Nature Science Foundation of China [20180114]
RADA16/CaSO4/HA composite biomaterials with controlled release of bFGF show enhanced in vitro osteogenic differentiation and in vivo bone formation, making it a promising injectable biomaterial for bone defect repair.
Injectable biomaterials obtain increasing attention for the repair of bone defect due to the advantage of defect margin adaptability and easy handling. Our previous studies found that RADA16 peptide hydrogel could significantly promote bone formation. In order to improve the mechanical force, RADA16 peptide hydrogel was used to fill the porous calcium sulfate/nano-hydroxyapatite (CaSO4/HA) cement biomaterial for the fabrication of RADA16/CaSO4/HA composite biomaterial. Especially, RADA16 peptide hydrogel had the ability to slowly release growth factors for bone regeneration. Here, RADA16/CaSO4/HA composite biomaterials afforded the controlled and sustainable release of basic fibroblast growth factor (bFGF) for more than 32 days. Compared to RADA16/CaSO4/HA biomaterial, RADA16 + bFGF/CaSO4/HA composite biomaterial could further improve in vitro osteogenic differentiation of MC3T3 cells as evidenced by the increased expression of osteogenic markers (i.e. runt-related transcription factor 2 [Runx2], osteocalcin [OCN] and alkaline phosphatase [ALP]) and in vivo bone formation of femoral condyle defects which were confirmed by histological staining and micro-CT analyses. These indicate that bFGF-incorporated RADA16/CaSO4/HA can further improve bone formation through the controlled release of bFGF, which provides a novel injectable biomaterial design to specifically release growth factors for bone defects.(c) 2022 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据