期刊
REDOX BIOLOGY
卷 48, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.redox.2021.102178
关键词
Ischemic stroke; Ischemia/reperfusion; Hypoxia/reoxygenation; In vivo optical brain interrogation; Genetically encoded fluorescent biosensors; Hydrogen peroxide
资金
- Russian Science Foundation (RSF) [17-1501175]
- Ministry of Science and Higher Education [075-15-2019-1933, 14.Z50.31.0040]
- Russian Foundation for Basic Research [18-29-20031, 19-02-00473]
- Russian Science Foundation [20-12-00088]
- Welch Foundation [A-1801-20180324]
- Russian Science Foundation [20-15-18021] Funding Source: Russian Science Foundation
Ischemic cerebral stroke is a major cause of death and disability in humans, but the molecular processes underlying its development are poorly understood. The study recorded the dynamics of intracellular pH and H2O2 during I/R and found a significant difference in the metabolic processes between in vivo and cultured neuron models. The findings highlight the limitations of cell culture as a predictor of metabolic events in vivo.
Ischemic cerebral stroke is one of the leading causes of death and disability in humans. However, molecular processes underlying the development of this pathology remain poorly understood. There are major gaps in our understanding of metabolic changes that occur in the brain tissue during the early stages of ischemia and reperfusion. In particular, it is generally accepted that both ischemia (I) and reperfusion (R) generate reactive oxygen species (ROS) that cause oxidative stress which is one of the main drivers of the pathology, although ROS generation during I/R was never demonstrated in vivo due to the lack of suitable methods. In the present study, we record for the first time the dynamics of intracellular pH and H2O2 during I/R in cultured neurons and during experimental stroke in rats using the latest generation of genetically encoded biosensors SypHer3s and HyPer7. We detect a buildup of powerful acidosis in the brain tissue that overlaps with the ischemic core from the first seconds of pathogenesis. At the same time, no significant H2O2 generation was found in the acute phase of ischemia/reperfusion. HyPer7 oxidation in the brain was detected only 24 h later. Comparison of in vivo experiments with studies on cultured neurons under I/R demonstrates that the dynamics of metabolic processes in these models significantly differ, suggesting that a cell culture is a poor predictor of metabolic events in vivo.
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