期刊
REDOX BIOLOGY
卷 48, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.redox.2021.102185
关键词
AMP-Activated protein kinase alpha 2; Autophagy; Cardiotoxicity; Doxorubicin; Epigallocatechin-3-gallate; Ferroptosis
资金
- Natural Science Foundation of China [82160685, 81803534, 81673431]
The mechanism of Doxorubicin-induced cardiotoxicity is complex, involving multiple cell death forms, with clinical intervention effect not ideal. By inhibiting iron accumulation, oxidative stress, and abnormal lipid metabolism, it is possible to alleviate Doxorubicin-induced cardiotoxicity-induced ferroptosis and protect the myocardium. Epigallocatechin-3-gallate is a potential candidate phytochemical for combating Doxorubicin-induced cardiotoxicity.
Reports indicate that the mechanism of doxorubicin (Dox)-induced cardiotoxicity is very complex, involving multiple regulatory cell death forms. Furthermore, the clinical intervention effect is not ideal. Iron dependence, abnormal lipid metabolism, and excess reactive oxygen species generation, three characteristics of ferroptosis, are potential therapeutic intervention targets. Here, we confirmed in vitro and in vivo that at least autophagy, apoptosis, and ferroptosis are involved in Dox cardiotoxicity-induced damage. When the neonatal rat cardiomyocytes and H9C2 cells or C57BL/6 mice were subjected to Dox-induced cardiotoxicity, epigallocatechin-3-gallate pretreatment could effectively decrease iron accumulation, inhibit oxidative stress and abnormal lipid metabolism, and thereby alleviate Dox cardiotoxicity-induced ferroptosis and protect the myocardium according to multiple functional, enzymatic, and morphological indices. The underlying mechanism was verified to involve the upregulation and activation of AMP-activated protein kinase alpha 2, which promoted adaptive autophagy, increased energy supply, and maintained mitochondrial function. We believe that epigallocatechin-3-gallate is a candidate phytochemical against Dox-induced cardiotoxicity.
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